University of Maryland LibrariesDigital Repository at the University of Maryland
    • Войти
    Просмотр элемента 
    •   Главная
    • A. James Clark School of Engineering
    • Fischell Department of Bioengineering
    • Fischell Department of Bioengineering Research Works
    • Просмотр элемента
    •   Главная
    • A. James Clark School of Engineering
    • Fischell Department of Bioengineering
    • Fischell Department of Bioengineering Research Works
    • Просмотр элемента
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by recombination

    Thumbnail
    Открыть
    Ammayappan, et al.pdf (427.8Kb)
    No. of downloads: 390

    Дата
    2008-12-22
    Автор
    Ammayappan, Arun
    Upadhyay, Chitra
    Gelb, Jack Jr.
    Vakharia, Vikram N
    Citation
    Ammayappan, A., Upadhyay, C., Gelb, J. J., & Vakharia, V. N. (January 01, 2008). Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by recombination. Virology Journal, 5.
    Metadata
    Показать полную информацию
    Аннотации
    An infectious bronchitis virus Arkansas DPI (Ark DPI) virulent strain was sequenced, analyzed and compared with many different IBV strains and coronaviruses. The genome of Ark DPI consists of 27,620 nucleotides, excluding poly (A) tail, and comprises ten open reading frames. Comparative sequence analysis of Ark DPI with other IBV strains shows striking similarity to the Conn, Gray, JMK, and Ark 99, which were circulating during that time period. Furthermore, comparison of the Ark genome with other coronaviruses demonstrates a close relationship to turkey coronavirus. Among non-structural genes, the 5'untranslated region (UTR), 3C-like proteinase (3CL pro) and the polymerase (RdRp) sequences are 100% identical to the Gray strain. Among structural genes, S1 has 97% identity with Ark 99; S2 has 100% identity with JMK and 96% to Conn; 3b 99%, and 3C to N is 100% identical to Conn strain. Possible recombination sites were found at the intergenic region of spike gene, 3'end of S1 and 3a gene. Independent recombination events may have occurred in the entire genome of Ark DPI, involving four different IBV strains, suggesting that genomic RNA recombination may occur in any part of the genome at number of sites. Hence, we speculate that the Ark DPI strain originated from the Conn strain, but diverged and evolved independently by point mutations and recombination between field strains.
    URI
    http://hdl.handle.net/1903/13387
    Collections
    • Fischell Department of Bioengineering Research Works

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    Просмотр

    Весь DSpaceСообщества и коллекцииДата публикацииАвторыНазванияТематикаЭта коллекцияДата публикацииАвторыНазванияТематика

    Моя учетная запись

    ВойтиРегистрация
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility