Skip to content
University of Maryland LibrariesDigital Repository at the University of Maryland
    • Login
    View Item 
    •   DRUM
    • University Libraries
    • Library Staff Research Works
    • Library Staff Research Works
    • View Item
    •   DRUM
    • University Libraries
    • Library Staff Research Works
    • Library Staff Research Works
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Arachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomes

    Thumbnail
    View/Open
    BaykoushevaArachidonicAcidJBC.pdf (578.3Kb)
    No. of downloads: 439

    Date
    1994
    Author
    Baykousheva, S. P.
    Luthria, D. L.
    Sprecher, H.
    Citation
    Baykousheva, S. P.; Luthria, D. L.; Sprecher, H., Arachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomes. Journal of Biological Chemistry 1994, 269 (28), 18390-18394.
    Metadata
    Show full item record
    Abstract
    Peroxisomal beta-oxidation of linoleic acid and arachidonic acid was depressed when 1-palmitoyl-sn-glycero-3-phosphocholine and microsomes were included in incubations. This reduction was due to the esterification of the substrate into the acceptor by microsomal 1-acyl-sn-glycero-3- phosphocholine acyltransferase. The first cycle of the beta-oxidation of 7,10,13,16-docosatetraenoic acid was independent of 1-acyl-sn-glycero-3-phosphocholine and microsomes. However, when arachidonate was produced it was esterified rather than serving as a substrate for continued beta-oxidation. When arachidonate and linoleate were incubated with peroxisomes alone, 2-trans-4,7,10-hexadecatetraenoic acid and 2-trans-4-decadienoic acid were the respective end products of beta-oxidation. 2-Oxo-8,11-heptadecadienone, a catabolite produced from linoleate, was most likely a nonenzymatic decarboxylation product of 3-oxo-9,12-octadecadienoic acid. In addition to the termination of beta-oxidation by microsomal-peroxisomal communication, our results with linoleate and arachidonate suggest that the reaction catalyzed by 2-trans-4-cis-dienoyl-CoA reductase is the control step in double bond removal. In addition, the beta-ketothiolase step may play a regulatory role in the peroxisomal beta-oxidation of linoleate but not arachidonate or 7,10,13,16-docosatetraenoic acid.
    Notes
    Available online at http://www.jbc.org/content/269/28/18390.full.pdf+html
    URI
    http://hdl.handle.net/1903/11419
    Collections
    • Library Staff Research Works

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    Browse

    All of DRUMCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility