Arachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomes
| dc.contributor.author | Baykousheva, S. P. | |
| dc.contributor.author | Luthria, D. L. | |
| dc.contributor.author | Sprecher, H. | |
| dc.date.accessioned | 2011-07-05T11:44:50Z | |
| dc.date.available | 2011-07-05T11:44:50Z | |
| dc.date.issued | 1994 | |
| dc.description | Available online at http://www.jbc.org/content/269/28/18390.full.pdf+html | en_US |
| dc.description.abstract | Peroxisomal beta-oxidation of linoleic acid and arachidonic acid was depressed when 1-palmitoyl-sn-glycero-3-phosphocholine and microsomes were included in incubations. This reduction was due to the esterification of the substrate into the acceptor by microsomal 1-acyl-sn-glycero-3- phosphocholine acyltransferase. The first cycle of the beta-oxidation of 7,10,13,16-docosatetraenoic acid was independent of 1-acyl-sn-glycero-3-phosphocholine and microsomes. However, when arachidonate was produced it was esterified rather than serving as a substrate for continued beta-oxidation. When arachidonate and linoleate were incubated with peroxisomes alone, 2-trans-4,7,10-hexadecatetraenoic acid and 2-trans-4-decadienoic acid were the respective end products of beta-oxidation. 2-Oxo-8,11-heptadecadienone, a catabolite produced from linoleate, was most likely a nonenzymatic decarboxylation product of 3-oxo-9,12-octadecadienoic acid. In addition to the termination of beta-oxidation by microsomal-peroxisomal communication, our results with linoleate and arachidonate suggest that the reaction catalyzed by 2-trans-4-cis-dienoyl-CoA reductase is the control step in double bond removal. In addition, the beta-ketothiolase step may play a regulatory role in the peroxisomal beta-oxidation of linoleate but not arachidonate or 7,10,13,16-docosatetraenoic acid. | en_US |
| dc.identifier.citation | Baykousheva, S. P.; Luthria, D. L.; Sprecher, H., Arachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomes. Journal of Biological Chemistry 1994, 269 (28), 18390-18394. | en_US |
| dc.identifier.issn | 0021-9258 | |
| dc.identifier.other | http://www.jbc.org/content/269/28/18390.full.pdf+html | |
| dc.identifier.uri | http://hdl.handle.net/1903/11419 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. | en_US |
| dc.relation.isAvailableAt | Digital Repository at the University of Maryland | en_us |
| dc.relation.isAvailableAt | University of Maryland Libraries | en_us |
| dc.relation.isAvailableAt | University of Maryland (College Park, MD) | en_us |
| dc.subject | Baykousheva | en_US |
| dc.subject | Baykoucheva | en_US |
| dc.subject | arachidonic acid | en_US |
| dc.subject | Mass spectrometry | en_US |
| dc.subject | Microsomes | en_US |
| dc.subject | Liver | en_US |
| dc.subject | Rats | en_US |
| dc.subject | Metabolism | en_US |
| dc.title | Arachidonic acid formed by peroxisomal β-oxidation of 7,10,13,16- docosatetraenoic acid is esterified into 1-acyl-sn-glycero-3-phosphocholine by microsomes | en_US |
| dc.type | Article | en_US |