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    A study of unusual metabolic variants of <italic>Aeromonas caviae</italic> and <italic>Aeromonas hydrophila</italic> using a polyphasic taxonomic approach

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    Date
    2010
    Author
    Chang, Zenas
    Advisor
    Joseph, Sam W
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    Abstract
    Variation in acid production from carbohydrate metabolism has been identified in <italic>Aeromonas</italic> as a potential indicator for new subspecies. Therefore, pure cultures of non-lactose fermenting <italic>Aeromonas caviae</italic>, a cause of waterborne infections in humans and other vertebrates, were studied after noting a mixture of acid producing and non-acid producing colonies after four days of incubation on MacConkey agar at ambient temperature. Unusual arabinose negative strains of <italic>A. hydrophila</italic> (usually arabinose positive) were added to the project to further study the correlation between carbohydrate fermentation and taxonomy. These metabolic variants of <italic>A. caviae</italic> and <italic>A. hydrophila</italic> were studied for phenotypic differences via carbohydrate utilization assays as well as genotypic differences via FAFLP. The results suggest that the <italic>A. caviae</italic> isolates MB3 and MB7 should be considered novel subspecies, while the arabinose negative strain designated <italic>A. hydrophila</italic> subsp. <italic>dhakensis</italic> is correctly identified as a subspecies of <italic>A. hydrophila</italic>.
    URI
    http://hdl.handle.net/1903/10962
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    • Cell Biology & Molecular Genetics Theses and Dissertations
    • UMD Theses and Dissertations

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    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
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