A study of unusual metabolic variants of <italic>Aeromonas caviae</italic> and <italic>Aeromonas hydrophila</italic> using a polyphasic taxonomic approach

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dc.contributor.advisorJoseph, Sam Wen_US
dc.contributor.authorChang, Zenasen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2010-10-07T06:14:05Z
dc.date.available2010-10-07T06:14:05Z
dc.date.issued2010en_US
dc.description.abstractVariation in acid production from carbohydrate metabolism has been identified in <italic>Aeromonas</italic> as a potential indicator for new subspecies. Therefore, pure cultures of non-lactose fermenting <italic>Aeromonas caviae</italic>, a cause of waterborne infections in humans and other vertebrates, were studied after noting a mixture of acid producing and non-acid producing colonies after four days of incubation on MacConkey agar at ambient temperature. Unusual arabinose negative strains of <italic>A. hydrophila</italic> (usually arabinose positive) were added to the project to further study the correlation between carbohydrate fermentation and taxonomy. These metabolic variants of <italic>A. caviae</italic> and <italic>A. hydrophila</italic> were studied for phenotypic differences via carbohydrate utilization assays as well as genotypic differences via FAFLP. The results suggest that the <italic>A. caviae</italic> isolates MB3 and MB7 should be considered novel subspecies, while the arabinose negative strain designated <italic>A. hydrophila</italic> subsp. <italic>dhakensis</italic> is correctly identified as a subspecies of <italic>A. hydrophila</italic>.en_US
dc.identifier.urihttp://hdl.handle.net/1903/10962
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.subject.pqcontrolledBiology, Geneticsen_US
dc.subject.pqcontrolledBiology, Molecularen_US
dc.subject.pquncontrolledAeromonasen_US
dc.subject.pquncontrolledAFLPen_US
dc.subject.pquncontrolledcaviaeen_US
dc.subject.pquncontrolledhydrophilaen_US
dc.subject.pquncontrolledsubspeciesen_US
dc.subject.pquncontrolledtaxonomyen_US
dc.titleA study of unusual metabolic variants of <italic>Aeromonas caviae</italic> and <italic>Aeromonas hydrophila</italic> using a polyphasic taxonomic approachen_US
dc.typeThesisen_US

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