Skip to content
University of Maryland LibrariesDigital Repository at the University of Maryland
    • Login
    View Item 
    •   DRUM
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • View Item
    •   DRUM
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Evaluation of the transcription of small RNA SgrS and glucose transporter mRNA ptsG in E. coli B and E. coli K cultures under high glucose conditions

    Thumbnail
    View/Open
    Ng_umd_0117N_11004.pdf (854.4Kb)
    No. of downloads: 656

    Date
    2009
    Author
    Ng, Weng Ian
    Advisor
    Wang, Nam Sun
    Metadata
    Show full item record
    Abstract
    <italic>Escherichia coli</italic> is commonly used as the production system for recombinant proteins. However, acetate accumulation in fermentation affects cell growth and protein yield. Recent studies have showed that the small RNA SgrS regulates the major glucose transporter mRNA <italic>ptsG</italic> in a post&ndash;transcriptional manner when the metabolic intermediate glucose&ndash;6&ndash;phosphate is accumulated intracellularly in <italic>E. coli</italic> K. Here, comparative analysis of the transcription of SgrS and <italic>ptsG</italic> is performed between <italic>E. coli</italic> B and <italic>E. coli</italic> K cultures in both shake flasks and bioreactor. Both strains expressed SgrS when grown on the non&ndash;metabolizable glucose analog &alpha;&ndash;methyl&ndash;glucoside. However, under high glucose conditions, only <italic>E. coli</italic> B showed significant expression of SgrS. This behavior is unaffected by oxygen supply and pH control. <italic>E. coli</italic> B produced less acetate on glucose than <italic>E. coli</italic> K in the bioreactor settings. This provides evidence of a possible connection between SgrS and acetate production in aerobic fermentation of <italic>E. coli</italic>.
    URI
    http://hdl.handle.net/1903/10023
    Collections
    • Chemical and Biomolecular Engineering Theses and Dissertations
    • UMD Theses and Dissertations

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    Browse

    All of DRUMCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility