Evaluation of the transcription of small RNA SgrS and glucose transporter mRNA ptsG in E. coli B and E. coli K cultures under high glucose conditions

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dc.contributor.advisorWang, Nam Sunen_US
dc.contributor.authorNg, Weng Ianen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2010-02-19T07:14:02Z
dc.date.available2010-02-19T07:14:02Z
dc.date.issued2009en_US
dc.description.abstract<italic>Escherichia coli</italic> is commonly used as the production system for recombinant proteins. However, acetate accumulation in fermentation affects cell growth and protein yield. Recent studies have showed that the small RNA SgrS regulates the major glucose transporter mRNA <italic>ptsG</italic> in a post&ndash;transcriptional manner when the metabolic intermediate glucose&ndash;6&ndash;phosphate is accumulated intracellularly in <italic>E. coli</italic> K. Here, comparative analysis of the transcription of SgrS and <italic>ptsG</italic> is performed between <italic>E. coli</italic> B and <italic>E. coli</italic> K cultures in both shake flasks and bioreactor. Both strains expressed SgrS when grown on the non&ndash;metabolizable glucose analog &alpha;&ndash;methyl&ndash;glucoside. However, under high glucose conditions, only <italic>E. coli</italic> B showed significant expression of SgrS. This behavior is unaffected by oxygen supply and pH control. <italic>E. coli</italic> B produced less acetate on glucose than <italic>E. coli</italic> K in the bioreactor settings. This provides evidence of a possible connection between SgrS and acetate production in aerobic fermentation of <italic>E. coli</italic>.en_US
dc.identifier.urihttp://hdl.handle.net/1903/10023
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.subject.pquncontrolledEscherichia colien_US
dc.subject.pquncontrolledglucose uptakeen_US
dc.subject.pquncontrolledpost-transcriptional regulationen_US
dc.subject.pquncontrolledPTSen_US
dc.subject.pquncontrolledSgrS-ptsG mechanismen_US
dc.subject.pquncontrolledsmall RNAen_US
dc.titleEvaluation of the transcription of small RNA SgrS and glucose transporter mRNA ptsG in E. coli B and E. coli K cultures under high glucose conditionsen_US
dc.typeThesisen_US

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