Office of Undergraduate Research

Permanent URI for this communityhttp://hdl.handle.net/1903/20157

Emphasizing equitable and inclusive access to research opportunities, the University of Maryland's Office of Undergraduate Research (OUR) empowers undergraduates and faculty to engage and succeed in inquiry, creative activity, and scholarship. This collection includes materials shared by undergraduate researchers during OUR events. It also encompasses materials from Undergraduate Research Day 2020, Undergraduate Research Day 2021, and Undergraduate Research Day 2022, which were organized by the Maryland Center for Undergraduate Research.

Browse

Author: search.filters.author.Bogusch, AlejandraSubject: search.filters.subject.First-Year Innovation and Research Experience (FIRE)

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    One-Pot Ligation LAMP Assay to Detect miRNA-222: A Glioma Biomarker
    (2024) Adane, Yedidya; Anjum, Azkah; Bogusch, Alejandra; Fernandes, Catarina; Naseem, Zoya; Pallavajjala, Roshni; Vempaty, Nitya; Spirito, Catherine
    Modern day cancer diagnostic methods are invasive, time-consuming, expensive, and inaccessible to most. Delayed cancer diagnosis can lower patient survival rates. By designing a Ligation Loop-Mediated Isothermal Amplification (Ligation LAMP) assay, an alternative amplification method to PCR, we can detect miRNA-222: an established biomarker that is found in elevated levels in the bloodstream of early-stage glioma patients. Employing an experimental design, this study confirms that miRNA-222 elicits a detectable colorimetric and fluorescent output through the Ligation LAMP assay, demonstrating its specificity and sensitivity to our biomarker. We are currently working on implementing our assay into a one-pot system, using Thermally Responsive Alkane Partitions (TRAPs). This innovative approach may transform current diagnostic tools and thus increase glioma survival rates.