Plant Science & Landscape Architecture Theses and Dissertations
Permanent URI for this collectionhttp://hdl.handle.net/1903/2797
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Item Nitrogen cycling by grass-brassica mixtures in the Mid-Atlantic(2019) Gaimaro, Joshua Ruben; Tully, Kate; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Mixtures of cover crop species may be more effective than monocultures at internal nutrient cycling due to their ability to occupy different niches. Our study investigates nitrogen (N) cycling of radish (Raphanus sativus L.) and rye (Secale cereal L.) in monocultures and mixtures compared to a no cover crop control. The study was established on fine-textured soils near Laurel, MD where we estimated N leaching losses, quantified mineral soil N (to 60 cm), and cover crop biomass N for two years. Forage radish suppressed estimated N leaching in the fall, while cereal rye suppressed estimated N leaching in the spring. In this study, growing radish in a mixture with rye decreased the risk of N leaching losses and enhanced N cycling due to the difference in timing of N uptake and release. Our research indicates that grass-brassica mixtures are a flexible management tool for mitigating N leaching in the Mid-Atlantic.Item Influence of Nitrogen and Sink Competition on Shoot Growth of Poplar(2016) Egekwu, Chioma; Coleman, Gary D; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Terrestrial and oceanic biomass carbon sinks help reduce anthropogenic CO2 emissions and mitigate the long-term effect of increasing atmospheric CO2. Woody plants have large carbon pools because of their long residence time, however N availability can negatively impact tree responses to elevated CO2. Seasonal cycling of internal N in trees is a component that contributes to fitness especially in N limited environments. It involves resorption from senescing leaves of deciduous trees and storage as vegetative storage proteins (VSP) in perennial organs. Populus is a model organism for tree biology that efficiently recycles N. Bark storage proteins (BSP) are the most abundant VSP that serves as seasonal N reserves. Here I show how poplar growth is influenced by N availability and how growth is influenced by shoot competition for stored N reserves. I also provide data that indicates that auxin mediates BSP catabolism during renewed shoot growth. Understanding the components of N accumulation, remobilization and utilization can provide insights leading to increasing N use efficiency (NUE) of perennial plants.Item NITROGEN REMOBILIZATION AND THE NUCLEOSIDE PHOSPHORYLASE-LIKE VEGETATIVE STORAGE PROTEIN FAMILY IN POPULUS: CHARACTERIZATION, REGULATION AND TRANSGENES(2012) Pettengill, Emily Ann; Coleman, Gary D; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Nutrient remobilization and storage allow plants to direct resources toward growth, maintenance and reproduction and redirect nutrients in response to environmental conditions or stresses. Particularly for perennial plants, these capabilities are critical to surviving periods of unfavorable growth such as winter and nutrient limited environments. In Populus, bark storage proteins (BSPs) have a dominant role in seasonal storage, and proteins related to BSPs, known as nucleoside phosphorylase-like (NP-like) proteins, can also participate in short-term storage. This research presents a comprehensive examination of the NP-like gene family by characterizing their expression, exploring evolutionary relationships within the plant kingdom and investigating metabolic regulation. I also developed and tested a set of qPCR reference genes to use for data normalization in two Populus species and four tissue-types. Lastly, transgenic trees were created to investigate the developmental or physiological functions of altered levels of BSP. Experiments characterizing the spatial and temporal expression of NP-like genes implicated a functional role for all members. Those results also support the phylogenetic analyses demonstrating the expansion of the gene family, which may have occurred through subfunctionalization. I also examined the regulation of carbon (C) and nitrogen (N) metabolites on the NP-like gene family expression and observed that amino acids, N compounds and gamma-aminobutyric acid (GABA) treatments modulate expression and likely have a role in regulatory pathways. By investigating transgenic trees with altered BSP levels, I present preliminary evidence that BSPs may have a role in nutrient signaling capable of modulating photosynthesis in young leaves. The results of this work deepen our understanding of nutrient remobilization and storage in Populus on regulatory, evolutionary and functional levels. Practically, the results can advance efforts to increase N use efficiency for sustainable biomass increases in Populus for use in agro-forestry, as biofuel feedstock, in phytoremediation and for carbon sequestration.Item Modeling Nitrogen, Phosphorus and Water Dynamics in Greenhouse and Nursery Production Systems(2011) Majsztrik, John Christopher; Lea-Cox, John D.; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Nutrient and sediment runoff from the six states and Washington, DC that form the Chesapeake Bay watershed is a major cause of environmental degradation in the Bay and its tributaries. Agriculture contributes a substantial portion of these non-point source loads that reach the Bay from its tributaries. Research in this area has traditionally focused on agronomic farm contributions, with limited research on the nursery and greenhouse industry. This research presents the first known attempt to model operation-specific information, validated by published research data, where multiple variables are assessed simultaneously. This research provides growers and researchers with a tool to assess and understand the cultural and environmental impact of current practices, and predict the impact of improving those practices. Separate models were developed for greenhouse, container-nursery and field-nursery operations, since specific production variables and management practices vary. Each model allows for simple entry of production input variables, which interface with the Stella modeling layer. Each model was first calibrated with one published research study, and subsequently validated with another peer-reviewed study, with multiple independent runs for each model. Validation results for all three models showed consistent agreement between model outputs and published results, increasing confidence that models accurately process all input data. Verified models were then used to run a number of what-if scenarios, based upon a database of production practices that was gathered from 48 nursery and greenhouse operations in Maryland. This database provided a detailed analysis of current practices in Maryland, and adds significantly to our understanding of various operational practices in these horticultural industries. Results of the what-if scenarios highlighted model sensitivities and provided answers to hypotheses developed from the analysis of the management database. Some model functions, such as denitrification, would greatly benefit from additional research and further model modification. Models were designed to be easily adapted to local conditions for use throughout the U.S. and potentially other parts of the world.Item Corn Residual Nitrate and its Implications for Fall Nitrogen Management in Winter Wheat(2011) Forrestal, Patrick Joseph; Kratochvil, Robert J; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Corn (Zea mays, L.) production typically requires supplemental nitrogen (N) to optimize yields. In dryland corn production systems, where N is applied during the early to mid-vegetative growth stages, inappropriate N applications or limited moisture during the growing season can result in large disparities between optimum and applied N rates. This leads to variable post-harvest residual nitrate (NO3-N) accumulation, which is susceptible to loss. However, this NO3-N could provide the starter N requirement of the subsequent winter wheat (Triticum aestivum, L.) crop. Accounting for residual NO3-N present at wheat planting is important to avoid compounding N loss potential due to corn residual NO3-N accumulation. The objectives of this study were to 1) examine plant based tools for assessing soil NO3-N; 2) to examine post-harvest residual NO3-N accumulation patterns following corn production; 3) to determine optimum fall starter N rates for winter wheat production; and 4) to identify a soil NO3-N level above which starter N could be forgone without negative agronomic effect. This study found that plant canopy measurements are useful tools for assessing corn N management and for identifying drought sites, which had the greatest NO3-N accumulations. The corn stalk nitrate test was significantly (p<0.001) and positively correlated with soil residual NO3-N (r2=0.41). Greatest soil residual NO3-N accumulation occurred where drought conditions reduced production. The agronomic optimum fall starter N rate for winter wheat in Maryland is 17 to 34 kg N ha-1 where soil NO3-N concentration to 15 cm depth is less than 15 mg kg-1. However, the fall starter N response was highly variable and declined significantly (p<0.01) as fall precipitation after planting increased. The results of this study indicate that residual NO3-N levels at planting should be considered before applying fall starter N to winter wheat.