Undergraduate Research Day 2021

Permanent URI for this collectionhttp://hdl.handle.net/1903/27016

With students involved in so many research opportunities, Undergraduate Research Day provides the perfect opportunity for them to share their work with the campus community. Held each April, Undergraduate Research Day showcases current research, scholarship, and artistic endeavors.

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Author: search.filters.author.Baker, CharleySubject: search.filters.subject.Bioinformatics in Microbiology

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    Identification of Clostridium Phage Endolysins with Novel Multimeric Genetic Sequences
    (2021) Bokil, Eesha; Baker, Charley; Nelson, Daniel; O'Hara, Jessica
    The endolysin CD27L is produced by the Clostridium phage phiCD27. This phage targets the bacteria and uses the endolysin’s enzymatic properties to lyse cells from within and release new replicated phages. Past studies have characterized the two domains of CD27L’s genetic sequence, the enzymatically active domain (EAD) at the N-terminus and the cell wall binding domain (CBD) at the C-terminus connected by a linker sequence. The gene sequence order is EAD-linker-CBD. A unique aspect of CD27L is its ability to form a multimeric enzymatic structure from these two domains where one EAD and multiple CBDs are present in one structure. This multimeric endolysin is formed from one gene, so translation of the one sequence uses two ribosome binding sites and two start codons. One ribosome binding site and start codon is before the EAD and the other in the linker sequence before the CBD. Our goal is to analyze the sequences of other Clostridium phage endolysins to find multimeric endolysins similar to CD27L. We are specifically looking for multiple ribosome binding sites with start codons or alternate start codons downstream in close proximity on one gene sequence.