MAKING APPLES BLUSH: UNDERSTANDING HOW THE COMBINED USE OF REFLECTIVE GROUNDCOVERS AND PLANT GROWTH REGULATORS IMPACT RED SKIN COLORATION AND QUALITY OF ‘HONEYCRISP’ APPLES IN THE MID-ATLANTIC US

Abstract

Apples are among the most valuable fruits globally, with 'Honeycrisp' ranking as the top sales-producing cultivar in the US. However, challenges such as insufficient red skin coloration and increased preharvest fruit drop significantly diminish their market value. Reflective groundcovers have been reported to enhance apple skin coloration, while the application of the plant growth regulator AVG reduces fruit drop but may negatively impact skin coloration. Research on the impacts of these practices in mid-Atlantic US-grown apples remains limited. In this two years study, our aims were 1) to evaluate the effect of reflective groundcovers on solar radiation (PPFD, UV) distribution; 2) to assess the combined effect of reflective groundcovers and ethylene inhibitor (AVG) on preharvest fruit drop, ethylene production, red blush percentage, and overall fruit quality; 3) to investigate the combined effect of reflective groundcovers and ethylene inhibitor (AVG) on expression level of key anthocyanin and ethylene biosynthesis related genes; 4) to determine the combined effect of reflective groundcovers and ethylene inhibitors (AVG) in the accumulation of total anthocyanin. Apples underwent four treatment combinations of reflective groundcover (Extenday) and AVG (130 mg L−1). Our findings revealed that Extenday significantly enhanced skin coloration (>75% blush) through increased reflectance of PPFD and UV radiation, along with increased IEC, while also accelerating fruit maturity, i.e., overripening. In fact, Extenday-only treated fruit exhibited the highest upregulation of ethylene and anthocyanin biosynthetic-related genes, as well as total anthocyanins. Conversely, AVG notably reduced fruit drop and decreased IEC, delaying fruit maturity while significantly diminishing red coloration (30–48% blush). AVG treated fruit significantly suppressed the expression of key ethylene and anthocyanin biosynthetic structural and regulatory genes, as well as total anthocyanins. The combined application of Extenday and AVG synergistically decreased fruit drop while enhancing skin coloration (>50% blush), but without inducing overripening. This combination fine-tuned the transcript accumulation of ethylene and anthocyanin biosynthetic-related genes, as well as total anthocyanins, enabling 'Honeycrisp' fruit to exceed 50% blush, while moderately increasing IEC (compared to Extenday-only and control fruit), thus enhancing fruit economic value. Therefore, combining Extenday and AVG can boost the market value for 'Honeycrisp' apples in the mid-Atlantic US.