Development of Carbon Nanotube Field-Effect Transistor Arrays for Detection of HER2 Overexpression in Breast Cancer

Simple item page
dc.contributor.advisorGomez, Romel Den_US
dc.contributor.authorAschenbach, Konrad Hsuen_US
dc.contributor.departmentElectrical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2011-07-06T05:48:12Z
dc.date.available2011-07-06T05:48:12Z
dc.date.issued2011en_US
dc.description.abstractWe developed a carbon nanotube biosensor platform that was deployed at the National Cancer Institute and successfully detected the HER2 oncogene in real cancer cells at clinically relevant levels. HER2 is a receptor protein that resides on the surface of certain cancer cells and is associated with higher aggressiveness in breast cancers. Overabundance of HER2 at the chromosomal, cell surface, and intermediate gene expression levels can all indicate a dangerous HER2 status. At the present, testing for HER2 status requires labor-intensive laboratory procedures using expensive reagents. Cost remains the major barrier to widespread screening. We propose an integrated electronic testing platform based on direct label-free gene detection. The system would integrate the various labor-intensive processes that are usually performed by skilled laboratory technicians. The heart of the system is an array of carbon nanotube field-effect transistors that can detect unlabelled nucleic acids via their intrinsic electric charges. We developed a scalable fabrication technique for carbon nanotube biosensor arrays, hardware and software for data acquisition and analysis, theoretical models for detection mechanism, and protocols for immobilization of peptide nucleic acid probes and hybridization of nucleic acids extracted from cells. We demonstrated detection of HER2 from real cell lines which express cancer genes, thereby lowering the technological barrier towards commercialization of a low-cost gene expression biosensor. The system is suitable for lab-on-a-chip integration, which could bring rapid, low-cost cancer diagnoses into the clinical setting.en_US
dc.identifier.urihttp://hdl.handle.net/1903/11501
dc.subject.pqcontrolledElectrical Engineeringen_US
dc.subject.pqcontrolledMolecular Biologyen_US
dc.subject.pqcontrolledMedicineen_US
dc.subject.pquncontrolledbiosensoren_US
dc.subject.pquncontrolledbreast canceren_US
dc.subject.pquncontrolledcarbon nanotubeen_US
dc.subject.pquncontrolledfield-effecten_US
dc.subject.pquncontrolledHER2en_US
dc.subject.pquncontrolledmRNAen_US
dc.titleDevelopment of Carbon Nanotube Field-Effect Transistor Arrays for Detection of HER2 Overexpression in Breast Canceren_US
dc.typeDissertationen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Aschenbach_umd_0117E_11997.pdf
Size:
3.17 MB
Format:
Adobe Portable Document Format
Download

Collections

UMD Theses and Dissertations
Electrical & Computer Engineering Theses and Dissertations