Genomic survey of the ectoparasitic mite Varroa destructor, a major pest of the honey bee Apis mellifera

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dc.contributor.authorCornman, R Scott
dc.contributor.authorSchatz, Michael C
dc.contributor.authorJohnston, J Spencer
dc.contributor.authorChen, Yan-Ping
dc.contributor.authorPettis, Jeff
dc.contributor.authorHunt, Greg
dc.contributor.authorBourgeois, Lanie
dc.contributor.authorElsik, Chris
dc.contributor.authorAnderson, Denis
dc.contributor.authorGrozinger, Christina M
dc.contributor.authorEvans, Jay D
dc.date.accessioned2021-11-15T15:52:35Z
dc.date.available2021-11-15T15:52:35Z
dc.date.issued2010-10-25
dc.description.abstractThe ectoparasitic mite Varroa destructor has emerged as the primary pest of domestic honey bees (Apis mellifera). Here we present an initial survey of the V. destructor genome carried out to advance our understanding of Varroa biology and to identify new avenues for mite control. This sequence survey provides immediate resources for molecular and population-genetic analyses of Varroa-Apis interactions and defines the challenges ahead for a comprehensive Varroa genome project. The genome size was estimated by flow cytometry to be 565 Mbp, larger than most sequenced insects but modest relative to some other Acari. Genomic DNA pooled from ~1,000 mites was sequenced to 4.3× coverage with 454 pyrosequencing. The 2.4 Gbp of sequencing reads were assembled into 184,094 contigs with an N50 of 2,262 bp, totaling 294 Mbp of sequence after filtering. Genic sequences with homology to other eukaryotic genomes were identified on 13,031 of these contigs, totaling 31.3 Mbp. Alignment of protein sequence blocks conserved among V. destructor and four other arthropod genomes indicated a higher level of sequence divergence within this mite lineage relative to the tick Ixodes scapularis. A number of microbes potentially associated with V. destructor were identified in the sequence survey, including ~300 Kbp of sequence deriving from one or more bacterial species of the Actinomycetales. The presence of this bacterium was confirmed in individual mites by PCR assay, but varied significantly by age and sex of mites. Fragments of a novel virus related to the Baculoviridae were also identified in the survey. The rate of single nucleotide polymorphisms (SNPs) in the pooled mites was estimated to be 6.2 × 10-5per bp, a low rate consistent with the historical demography and life history of the species. This survey has provided general tools for the research community and novel directions for investigating the biology and control of Varroa mites. Ongoing development of Varroa genomic resources will be a boon for comparative genomics of under-represented arthropods, and will further enhance the honey bee and its associated pathogens as a model system for studying host-pathogen interactions.en_US
dc.description.urihttps://doi.org/10.1186/1471-2164-11-602
dc.identifierhttps://doi.org/10.13016/molc-5yxe
dc.identifier.citationCornman, R.S., Schatz, M.C., Johnston, J.S. et al. Genomic survey of the ectoparasitic mite Varroa destructor, a major pest of the honey bee Apis mellifera. BMC Genomics 11, 602 (2010).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28110
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtBiologyen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectCodon Usageen_US
dc.subjectVarroa Miteen_US
dc.subjectMite Controlen_US
dc.subjectIndividual Miteen_US
dc.subjectAbundant Transposable Elementen_US
dc.titleGenomic survey of the ectoparasitic mite Varroa destructor, a major pest of the honey bee Apis melliferaen_US
dc.typeArticleen_US

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