B Cell Memory, CD23, and Lipid Metabolism: A Preliminary Study

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dc.contributor.advisorSong, Wenxiaen_US
dc.contributor.authorWiggins, Melvin Danielen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2013-02-07T06:38:50Z
dc.date.available2013-02-07T06:38:50Z
dc.date.issued2012en_US
dc.description.abstractWe each receive vaccinations throughout our lives, which protect us from many pathogens and gives long-term protection through generating a subset of memory lymphocytes. This study explores whether CD23 (Fcε receptor) and high fat diet have roles in regulating memory B cells. CD23 in B cells was examined using CD23 transgenic mice. My data show that, after antigenic stimulation, CD23 co-aggregates with the BCR. The percentages of isotype switched B cells as well as other peripheral B cell subsets in the spleen are not altered in unimmunized CD23 transgenic mice, implicating that CD23 does not have any significant role in the generation of memory B cells. High fat diet with and without high cholesterol led to increased numbers of mature follicular B cells and decreases in transitional B cells in a NPC1L1 independent manner.The marginal zone B cells numbers are increased in the mice fed high fat/high cholesterol diets. This suggests a possible role of high fat/high cholesterol diet in regulating the peripheral development of B cells.en_US
dc.identifier.urihttp://hdl.handle.net/1903/13597
dc.subject.pqcontrolledImmunologyen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pquncontrolledB Cellen_US
dc.subject.pquncontrolledCD23en_US
dc.subject.pquncontrolledFlow Cytometryen_US
dc.subject.pquncontrolledMemoryen_US
dc.subject.pquncontrolledObesityen_US
dc.subject.pquncontrolledRegulationen_US
dc.titleB Cell Memory, CD23, and Lipid Metabolism: A Preliminary Studyen_US
dc.typeThesisen_US

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