Microarray analysis of genes associated with cell surface NIS protein levels in breast cancer

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dc.contributor.authorBeyer, Sasha J
dc.contributor.authorZhang, Xiaoli
dc.contributor.authorJimenez, Rafael E
dc.contributor.authorLee, Mei-Ling T
dc.contributor.authorRichardson, Andrea L
dc.contributor.authorHuang, Kun
dc.contributor.authorJhiang, Sissy M
dc.date.accessioned2021-11-01T20:55:48Z
dc.date.available2021-11-01T20:55:48Z
dc.date.issued2011-10-11
dc.description.abstractNa+/I- symporter (NIS)-mediated iodide uptake allows radioiodine therapy for thyroid cancer. NIS is also expressed in breast tumors, raising potential for radionuclide therapy of breast cancer. However, NIS expression in most breast cancers is low and may not be sufficient for radionuclide therapy. We aimed to identify biomarkers associated with NIS expression such that mechanisms underlying NIS modulation in human breast tumors may be elucidated. Published oligonucleotide microarray data within the National Center for Biotechnology Information Gene Expression Omnibus database were analyzed to identify gene expression tightly correlated with NIS mRNA level among human breast tumors. NIS immunostaining was performed in a tissue microarray composed of 28 human breast tumors which had corresponding oligonucleotide microarray data available for each tumor such that gene expression associated w ith cell surface NIS protein level could be identified. NIS mRNA levels do not vary among breast tumors or when compared to normal breast tissues when detected by Affymetrix oligonucleotide microarray platforms. Cell surface NIS protein levels are much more variable than their corresponding NIS mRNA levels. Despite a limited number of breast tumors examined, our analysis identified cysteinyl-tRNA synthetase as a biomarker that is highly associated with cell surface NIS protein levels in the ER-positive breast cancer subtype. Further investigation on genes associated with cell surface NIS protein levels within each breast cancer molecular subtype may lead to novel targets for selectively increasing NIS expression/function in a subset of breast cancers patients.en_US
dc.description.urihttps://doi.org/10.1186/1756-0500-4-397
dc.identifierhttps://doi.org/10.13016/mydc-apdk
dc.identifier.citationBeyer, S.J., Zhang, X., Jimenez, R.E. et al. Microarray analysis of genes associated with cell surface NIS protein levels in breast cancer. BMC Res Notes 4, 397 (2011).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28084
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtEpidemiology & Biostatistics
dc.relation.isAvailableAtSchool of Public Health
dc.relation.isAvailableAtDigital Repository at the University of Maryland (DRUM)
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)
dc.subjectBreast Canceren_US
dc.subjectBreast Tumoren_US
dc.subjectOligonucleotide Microarrayen_US
dc.subjectBreast Cancer Molecular Subtypeen_US
dc.subjectCars Proteinen_US
dc.titleMicroarray analysis of genes associated with cell surface NIS protein levels in breast canceren_US
dc.typeArticleen_US

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