IN VITRO INDUCTION OF POLYPLOIDY IN CERCIS YUNNANENSIS HU ET CHENG

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dc.contributor.advisorColeman, Gary Den_US
dc.contributor.authorNadler, Joshua Danielen_US
dc.contributor.departmentPlant Science and Landscape Architecture (PSLA)en_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2010-02-19T06:40:44Z
dc.date.available2010-02-19T06:40:44Z
dc.date.issued2009en_US
dc.description.abstractMethods for in vitro induction of polyploid Cercis yunnanensis Hu et Cheng using oryzalin were developed and evaluated. Methods included treating either shoot or callus tissue for different exposure durations with either an aqueous solution of 150 micromolar oryzalin or the addition of oryzalin directly to solid media. Polyploid nuclei were determined by flow cytometry for all oryzalin treatments. Although the results indicate that most tissues measured were likely chimeras with respect to DNA content. Results indicate that treating shoot tissue with an aqueous solution of oryzalin for 12 to 96 hours produced tetraploid plants irrespective of the type of shoot explant treated. An unstable octaploid was formed from the treatment of a pre-cultured lateral shoot in an aqueous solution of oryzalin for 96 hours. In contrast shoots cultured on the solidified media failed to produce polyploid plants and there were no statistical differences between callus treatments regarding polyploid induction.en_US
dc.identifier.urihttp://hdl.handle.net/1903/9853
dc.subject.pqcontrolledAgriculture, Horticultureen_US
dc.subject.pquncontrolledCercis yunnanensisen_US
dc.subject.pquncontrolledflow cytometryen_US
dc.subject.pquncontrolledIn Vitroen_US
dc.subject.pquncontrolledOryzalinen_US
dc.subject.pquncontrolledPolyploiden_US
dc.titleIN VITRO INDUCTION OF POLYPLOIDY IN CERCIS YUNNANENSIS HU ET CHENGen_US
dc.typeThesisen_US

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