THERMODYNAMICS AND APPLICATION OF A PAIR OF SYNTHETIC NUCLEOBASES FROM ARTIFICIALLY EXPANDED GENETIC INFORMATION SYSTEM

dc.contributor.advisorKahn, Jason Den_US
dc.contributor.authorWang, Xiaoyuen_US
dc.contributor.departmentBiochemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2016-09-08T05:32:36Z
dc.date.available2016-09-08T05:32:36Z
dc.date.issued2016en_US
dc.description.abstractUV-melting experiments were performed on 9-mer duplexes containing a pair of synthetic nucleobases P·Z, two members of Expanded Genetic Information System (AEGIS), or P, Z containing mismatches. Enthalpy, entropy and free energy change were derived from simulation using two-state transition model. Nearest neighbor thermodynamic parameters of trimers or tetramers containing P·Z pair or P, Z containing mismatches were derived based on known nearest neighbor parameters. Proposed structures based on thermodynamic parameters are discussed. An application using P·Z pair as reverse selection tool of desired nucleic acid secondary structure is described.en_US
dc.identifierhttps://doi.org/10.13016/M2TB9Z
dc.identifier.urihttp://hdl.handle.net/1903/18690
dc.language.isoenen_US
dc.subject.pqcontrolledBiochemistryen_US
dc.subject.pqcontrolledBiophysicsen_US
dc.subject.pquncontrolledartificially expanded genetic information systemen_US
dc.subject.pquncontrollednearest neighboren_US
dc.subject.pquncontrolledsynthetic nucleobaseen_US
dc.subject.pquncontrolledthermodynamicsen_US
dc.subject.pquncontrolledUV meltingen_US
dc.titleTHERMODYNAMICS AND APPLICATION OF A PAIR OF SYNTHETIC NUCLEOBASES FROM ARTIFICIALLY EXPANDED GENETIC INFORMATION SYSTEMen_US
dc.typeDissertationen_US

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