INTERFERON-DEPENDENT AND -INDEPENDENT MODULATION OF WEST NILE VIRUS INFECTIONS OF HUMAN DERMAL FIBROBLASTS.

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dc.contributor.advisorFredericksen, Brenda Len_US
dc.contributor.authorHoover, Lisa Injaianen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2014-06-24T06:07:59Z
dc.date.available2014-06-24T06:07:59Z
dc.date.issued2014en_US
dc.description.abstractAlthough dermal fibroblasts are one of the first cell types exposed to West Nile virus (WNV) during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, I demonstrate that pathogenic, WNV-New York (WNV-NY), and nonpathogenic, WNV-Australia (WNV-AUS60) strains are able to infect and replicate in primary human dermal fibroblasts (HFFs). However, WNV-AUS60 replication and spread within HFFs was reduced compared to that of WNV-NY due to an interferon-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-&beta;-dependent reduction in particle infectivity. Overall, our data indicate that dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HFFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir <italic>in vivo</italic>.en_US
dc.identifier.urihttp://hdl.handle.net/1903/15319
dc.language.isoenen_US
dc.subject.pqcontrolledVirologyen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledfibroblasten_US
dc.subject.pquncontrolledinnate immune responseen_US
dc.subject.pquncontrolledinterferonen_US
dc.subject.pquncontrolledWest Nile virusen_US
dc.titleINTERFERON-DEPENDENT AND -INDEPENDENT MODULATION OF WEST NILE VIRUS INFECTIONS OF HUMAN DERMAL FIBROBLASTS.en_US
dc.typeDissertationen_US

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