CHARACTERIZATION OF THE MYO-INOSITOL (3) PHOSPHATE SYNTHASE GENE (MIPS) AND MAPPING OF A LPA MUTANT IN SOYBEAN (GLYCINE MAX (L.) MERRILL).

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dc.contributor.advisorKenworthy, William J.en_US
dc.contributor.advisorCosta, Jose M.en_US
dc.contributor.authorSalmon, Katherine Dianeen_US
dc.contributor.departmentPlant Science and Landscape Architecture (PSLA)en_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2004-08-27T05:41:38Z
dc.date.available2004-08-27T05:41:38Z
dc.date.issued2004-08-25en_US
dc.description.abstractLow phytic acid (LPA) is a mutation causing phosphorus to be stored as unbound phosphorus in the seed. LPA mutants show a high inorganic phosphorus (HIP) phenotype. Previous studies had indicated that LPA might be linked to the myo-inositol (3) phosphate synthase (MIPS) gene; this research attempted to associate a soybean HIP mutant with the MIPS gene. The parental and the F2 genotypes were tested in four ways: 1) SNP detection using the LCR protocol; 2) polymorphism detection with PCR; 3) high inorganic phosphorus (HIP) phenotype detection; and 4) oil and protein concentration. The two parental genotypes could not be differentiated in the LCR study. A PCR-based polymorphism was heritable in the F2 genotypes. HIP assay indicated multiple genes control the LPA mutant. A polymorphism was associated to the HIP phenotype. The three types of HIP phenotypes were not statistically different in oil and protein concentrations allowing implementation into a breeding program.en_US
dc.format.extent2064940 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/1852
dc.language.isoen_US
dc.subject.pqcontrolledAgriculture, Generalen_US
dc.subject.pqcontrolledBiology, Geneticsen_US
dc.titleCHARACTERIZATION OF THE MYO-INOSITOL (3) PHOSPHATE SYNTHASE GENE (MIPS) AND MAPPING OF A LPA MUTANT IN SOYBEAN (GLYCINE MAX (L.) MERRILL).en_US
dc.typeThesisen_US

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