Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae

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dc.contributor.authorRanjan, Peeyush
dc.contributor.authorKateriya, Suneel
dc.date.accessioned2021-07-12T17:42:52Z
dc.date.available2021-07-12T17:42:52Z
dc.date.issued2018-01-24
dc.description.abstractThe eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants.en_US
dc.description.urihttps://doi.org/10.1186/s13104-018-3181-4
dc.identifierhttps://doi.org/10.13016/xuor-xohf
dc.identifier.citationRanjan, P., Kateriya, S. Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae. BMC Res Notes 11, 65 (2018).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27336
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCell Biology & Molecular Geneticsen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectCoccomyxa subellipsoideaen_US
dc.subjectProton-pumping rhodopsinen_US
dc.subjectOptogeneticsen_US
dc.subjectLocalization of the microbial rhodopsin in eukaryotesen_US
dc.subjectChlorella vulgarisen_US
dc.titleLocalization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algaeen_US
dc.typeArticleen_US

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