The Transcriptional Regulator MphR(A): Characterization and Circuit Engineering
| dc.contributor.advisor | Cropp, T A | en_US |
| dc.contributor.author | Smolinsky, Adam Randall | en_US |
| dc.contributor.department | Chemistry | en_US |
| dc.contributor.publisher | Digital Repository at the University of Maryland | en_US |
| dc.contributor.publisher | University of Maryland (College Park, Md.) | en_US |
| dc.date.accessioned | 2008-06-20T05:38:52Z | |
| dc.date.available | 2008-06-20T05:38:52Z | |
| dc.date.issued | 2008-05-09 | en_US |
| dc.description.abstract | The MphR(A) transcriptional repressor protein was incorporated into high-efficiency ex-pression vectors, purified and characterized. Initial screens for crystallography solvents were conducted along with preliminary gel-shift and intrinsic fluorescence quenching ex-periments to obtain the equilibrium dissociation constant for the protein-DNA interac-tions. The constant was found to be 0.5 - 1.9 μM, depending on the method used. The gene was also incorporated into positive feedback circuits to detect macrolide antibiotics using various reporter genes and plasmid constructs. Qualitatively, the circuits showed a change in output upon the addition of MphR to the system. | en_US |
| dc.format.extent | 1127466 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1903/8229 | |
| dc.language.iso | en_US | |
| dc.subject.pqcontrolled | Chemistry, Biochemistry | en_US |
| dc.title | The Transcriptional Regulator MphR(A): Characterization and Circuit Engineering | en_US |
| dc.type | Thesis | en_US |
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