High throughput sequencing reveals novel and abiotic stress-regulated microRNAs in the inflorescences of rice

Simple item page
dc.contributor.authorBarrera-Figueroaroa, Blanca E
dc.contributor.authorGao, Lei
dc.contributor.authorWu, Zhigang
dc.contributor.authorZhou, Xuefeng
dc.contributor.authorZhu, Jianhua
dc.contributor.authorJin, Hailing
dc.contributor.authorLiu, Renyi
dc.contributor.authorZhu, Jian-Kang
dc.date.accessioned2021-09-30T17:00:21Z
dc.date.available2021-09-30T17:00:21Z
dc.date.issued2012-08-03
dc.description.abstractMicroRNAs (miRNAs) are small RNA molecules that play important regulatory roles in plant development and stress responses. Identification of stress-regulated miRNAs is crucial for understanding how plants respond to environmental stimuli. Abiotic stresses are one of the major factors that limit crop growth and yield. Whereas abiotic stress-regulated miRNAs have been identified in vegetative tissues in several plants, they are not well studied in reproductive tissues such as inflorescences. We used Illumina deep sequencing technology to sequence four small RNA libraries that were constructed from the inflorescences of rice plants that were grown under control condition and drought, cold, or salt stress. We identified 227 miRNAs that belong to 127 families, including 70 miRNAs that are not present in the miRBase. We validated 62 miRNAs (including 10 novel miRNAs) using published small RNA expression data in DCL1, DCL3, and RDR2 RNAi lines and confirmed 210 targets from 86 miRNAs using published degradome data. By comparing the expression levels of miRNAs, we identified 18, 15, and 10 miRNAs that were regulated by drought, cold and salt stress conditions, respectively. In addition, we identified 80 candidate miRNAs that originated from transposable elements or repeats, especially miniature inverted-repeat elements (MITEs). We discovered novel miRNAs and stress-regulated miRNAs that may play critical roles in stress response in rice inflorescences. Transposable elements or repeats, especially MITEs, are rich sources for miRNA origination.en_US
dc.description.urihttps://doi.org/10.1186/1471-2229-12-132
dc.identifierhttps://doi.org/10.13016/8bf4-ezko
dc.identifier.citationBarrera-Figueroa, B.E., Gao, L., Wu, Z. et al. High throughput sequencing reveals novel and abiotic stress-regulated microRNAs in the inflorescences of rice. BMC Plant Biol 12, 132 (2012).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28046
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtPlant Science & Landscape Architectureen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectAbiotic Stressen_US
dc.subjectSalt Stressen_US
dc.subjectMature miRNAsen_US
dc.subjectmiRNA Candidateen_US
dc.subjectmiRNA Predictionen_US
dc.titleHigh throughput sequencing reveals novel and abiotic stress-regulated microRNAs in the inflorescences of riceen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1471-2229-12-132.pdf
Size:
482.05 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.57 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Plant Science & Landscape Architecture Research Works