The Effect of Hyper-Osmotic Conditions on the Growth, Metabolism, and Specific Antibody Productivity of a GS-NS0 Cell Line

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dc.contributor.advisorWang, Nam Sen_US
dc.contributor.authorBrady, Stefanie Ellenen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2009-07-03T05:37:34Z
dc.date.available2009-07-03T05:37:34Z
dc.date.issued2009en_US
dc.description.abstractThe effect of cellular growth, metabolism, and monoclonal antibody production on an industrial GS-NS0 cell line to hyper-osmotic medium was studied. The GS-NS0 cell line was found to have an optimum growth rate at a medium osmolality of 350 mosm/kg and an optimum specific productivity at 450 mosm/kg. Medium osmolality was shown to affect cell size as the cell line exhibited a regulatory cell volume increase response after an initial introduction into hyper-osmotic conditions. The response of the cell line to an osmotic shift was also studied. Osmolality of the culture medium was increased, at two different time points, through the addition of NaCl. The shift in osmotic pressure was found to have a positive impact on specific productivity of the monoclonal antibody produced. A finger print of the metabolic response of the GS-NS0 cell line to increased medium osmolality was determined. The application of metabolomics to mammalian cell cultures has not been widely explored. In this study, the cells were quenched and extracted using methods previously developed for microbial and plant cultures. An increase in concentration of internal amino acids, known to be osmolytes, was found under hyper-osmotic conditions.en_US
dc.format.extent785851 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/9322
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pquncontrolledGS-NS0 Cell Cultureen_US
dc.subject.pquncontrolledmAb Productivityen_US
dc.subject.pquncontrolledMetabolomicsen_US
dc.subject.pquncontrolledOsmolarityen_US
dc.titleThe Effect of Hyper-Osmotic Conditions on the Growth, Metabolism, and Specific Antibody Productivity of a GS-NS0 Cell Lineen_US
dc.typeThesisen_US

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