Cooperativity and Steep Voltage Dependence in a Bacterial Channel

dc.contributor.authorLin, Shang H.
dc.contributor.authorChang, Kai-Ti
dc.contributor.authorCherian, Nuval
dc.contributor.authorWu, Benjamin
dc.contributor.authorPhee, Hyo
dc.contributor.authorCho, Christy
dc.contributor.authorColombini, Marco
dc.date.accessioned2023-11-14T15:06:27Z
dc.date.available2023-11-14T15:06:27Z
dc.date.issued2019-09-11
dc.description.abstractThis paper reports on the discovery of a novel three-membrane channel unit exhibiting very steep voltage dependence and strong cooperative behavior. It was reconstituted into planar phospholipid membranes formed by the monolayer method and studied under voltage-clamp conditions. The behavior of the novel channel-former, isolated from Escherichia coli, is consistent with a linearly organized three-channel unit displaying steep voltage-gating (a minimum of 14 charges in the voltage sensor) that rivals that of channels in mammalian excitable membranes. The channels also display strong cooperativity in that closure of the first channel permits the second to close and closure of the second channel permits closure of the third. All three have virtually the same conductance and selectivity, and yet the first and third close at positive potentials whereas the second closes at negative potentials. Thus, is it likely that the second channel-former is oriented in the membrane in a direction opposite to that of the other two. This novel structure is named “triplin.” The extraordinary behavior of triplin indicates that it must have important and as yet undefined physiological roles.
dc.description.urihttps://doi.org/10.3390/ijms20184501
dc.identifierhttps://doi.org/10.13016/dspace/9uuf-opt2
dc.identifier.citationLin, S.H.; Chang, K.-T.; Cherian, N.; Wu, B.; Phee, H.; Cho, C.; Colombini, M. Cooperativity and Steep Voltage Dependence in a Bacterial Channel. Int. J. Mol. Sci. 2019, 20, 4501.
dc.identifier.urihttp://hdl.handle.net/1903/31386
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtBiologyen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectEscherichia coli
dc.subjectplanar membrane
dc.subjectpore
dc.subjectdipole
dc.subjectelectrophysiology
dc.subjectvoltage gating
dc.titleCooperativity and Steep Voltage Dependence in a Bacterial Channel
dc.typeArticle
local.equitableAccessSubmissionNo

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