Three Clostridium species with Health Imparting Properties: In vitro Screening for Probiotic Potential
dc.contributor.advisor | Obanda, Diana | en_US |
dc.contributor.author | Mochama, Victor Moronge | en_US |
dc.contributor.department | Nutrition | en_US |
dc.contributor.publisher | Digital Repository at the University of Maryland | en_US |
dc.contributor.publisher | University of Maryland (College Park, Md.) | en_US |
dc.date.accessioned | 2024-09-23T06:33:28Z | |
dc.date.available | 2024-09-23T06:33:28Z | |
dc.date.issued | 2024 | en_US |
dc.description.abstract | This research aimed to unlock the probiotic potential of the genus Clostridium, which is often overshadowed by the predominant focus on pathogenic species. The study specifically targeted three promising Clostridium species: C. disporicum, C. celatum, and C. vincentii, which have shown potential in mitigating diet-induced obesity. Despite the challenges presented by the anaerobic growth requirements of Clostridium bacteria, the study capitalized on their capacity to sporulate. This characteristic provides an avenue to use them as probiotics, with resilient and dormant spores capable of surviving food processing and harsh stomach conditions. The resilience of these spores was examined by exposing them to oxygen, heat, gastrointestinal juices, and bile salts. The spores survived oxygen exposure, exhibited resilience to both bile salts and gastric acids, and demonstrated a survival temperature of 70°C. When exposed to suitable germination conditions in vitro, the spores successfully germinated. The study assessed the colonization potential of the bacteria by evaluating their adhesion ability, and all bacteria were found to have the adhesion ability. Furthermore, a safety assessment was conducted by examining hemolytic activity and antibiotic susceptibility to selected antibiotics. The bacteria were found to be susceptible to the antibiotics and did not exhibit hemolytic activity. Bile salt hydrolase (BSH) activity and antibacterial activities were also assessed, and none of the bacteria exhibited BSH activity or antibacterial activity. Antioxidant tests revealed that C. vincentii had the highest antioxidant properties. Assessment of anti-inflammatory properties showed that C. celatum downregulated the gene expression of cytokine inflammation markers IL-6, IL-1, and iNOS while upregulating TGF-β expression. In summary all 3 bacterial species showed good probiotic potential from the in vitro tests. Particularly the formation of resistant spores that later germinated to vegetative cells that produced molecular patterns with antioxidant and anti-inflammatory properties. This necessitates further studies on their probiotic properties. | en_US |
dc.identifier | https://doi.org/10.13016/i2dj-umjj | |
dc.identifier.uri | http://hdl.handle.net/1903/33479 | |
dc.language.iso | en | en_US |
dc.subject.pqcontrolled | Nutrition | en_US |
dc.subject.pqcontrolled | Molecular biology | en_US |
dc.subject.pqcontrolled | Microbiology | en_US |
dc.subject.pquncontrolled | Clostridium | en_US |
dc.subject.pquncontrolled | Health | en_US |
dc.subject.pquncontrolled | Microbiota | en_US |
dc.subject.pquncontrolled | Probiotics | en_US |
dc.subject.pquncontrolled | Resilience | en_US |
dc.subject.pquncontrolled | Spores | en_US |
dc.title | Three Clostridium species with Health Imparting Properties: In vitro Screening for Probiotic Potential | en_US |
dc.type | Thesis | en_US |
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