Three Clostridium species with Health Imparting Properties: In vitro Screening for Probiotic Potential

dc.contributor.advisorObanda, Dianaen_US
dc.contributor.authorMochama, Victor Morongeen_US
dc.contributor.departmentNutritionen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2024-09-23T06:33:28Z
dc.date.available2024-09-23T06:33:28Z
dc.date.issued2024en_US
dc.description.abstractThis research aimed to unlock the probiotic potential of the genus Clostridium, which is often overshadowed by the predominant focus on pathogenic species. The study specifically targeted three promising Clostridium species: C. disporicum, C. celatum, and C. vincentii, which have shown potential in mitigating diet-induced obesity. Despite the challenges presented by the anaerobic growth requirements of Clostridium bacteria, the study capitalized on their capacity to sporulate. This characteristic provides an avenue to use them as probiotics, with resilient and dormant spores capable of surviving food processing and harsh stomach conditions. The resilience of these spores was examined by exposing them to oxygen, heat, gastrointestinal juices, and bile salts. The spores survived oxygen exposure, exhibited resilience to both bile salts and gastric acids, and demonstrated a survival temperature of 70°C. When exposed to suitable germination conditions in vitro, the spores successfully germinated. The study assessed the colonization potential of the bacteria by evaluating their adhesion ability, and all bacteria were found to have the adhesion ability. Furthermore, a safety assessment was conducted by examining hemolytic activity and antibiotic susceptibility to selected antibiotics. The bacteria were found to be susceptible to the antibiotics and did not exhibit hemolytic activity. Bile salt hydrolase (BSH) activity and antibacterial activities were also assessed, and none of the bacteria exhibited BSH activity or antibacterial activity. Antioxidant tests revealed that C. vincentii had the highest antioxidant properties. Assessment of anti-inflammatory properties showed that C. celatum downregulated the gene expression of cytokine inflammation markers IL-6, IL-1, and iNOS while upregulating TGF-β expression. In summary all 3 bacterial species showed good probiotic potential from the in vitro tests. Particularly the formation of resistant spores that later germinated to vegetative cells that produced molecular patterns with antioxidant and anti-inflammatory properties. This necessitates further studies on their probiotic properties.en_US
dc.identifierhttps://doi.org/10.13016/i2dj-umjj
dc.identifier.urihttp://hdl.handle.net/1903/33479
dc.language.isoenen_US
dc.subject.pqcontrolledNutritionen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pquncontrolledClostridiumen_US
dc.subject.pquncontrolledHealthen_US
dc.subject.pquncontrolledMicrobiotaen_US
dc.subject.pquncontrolledProbioticsen_US
dc.subject.pquncontrolledResilienceen_US
dc.subject.pquncontrolledSporesen_US
dc.titleThree Clostridium species with Health Imparting Properties: In vitro Screening for Probiotic Potentialen_US
dc.typeThesisen_US

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