Genomic characterization of the Yersinia genus

dc.contributor.authorChen, Peter E
dc.contributor.authorCook, Christopher
dc.contributor.authorStewart, Andrew C
dc.contributor.authorNagarajan, Niranjan
dc.contributor.authorSommer, Dan D
dc.contributor.authorPop, Mihai
dc.contributor.authorThomason, Brendan
dc.contributor.authorThomason, Maureen P Kiley
dc.contributor.authorLentz, Shannon
dc.contributor.authorNolan, Nichole
dc.contributor.authorSozhamannan, Shanmuga
dc.contributor.authorSulakvelidze, Alexander
dc.contributor.authorMateczun, Alfred
dc.contributor.authorDu, Lei
dc.contributor.authorZwick, Michael E
dc.contributor.authorRead, Timothy D
dc.description.abstractNew DNA sequencing technologies have enabled detailed comparative genomic analyses of entire genera of bacterial pathogens. Prior to this study, three species of the enterobacterial genus Yersinia that cause invasive human diseases (Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica) had been sequenced. However, there were no genomic data on the Yersinia species with more limited virulence potential, frequently found in soil and water environments. We used high-throughput sequencing-by-synthesis instruments to obtain 25- to 42-fold average redundancy, whole-genome shotgun data from the type strains of eight species: Y. aldovae, Y. bercovieri, Y. frederiksenii, Y. kristensenii, Y. intermedia, Y. mollaretii, Y. rohdei, and Y. ruckeri. The deepest branching species in the genus, Y. ruckeri, causative agent of red mouth disease in fish, has the smallest genome (3.7 Mb), although it shares the same core set of approximately 2,500 genes as the other members of the species, whose genomes range in size from 4.3 to 4.8 Mb. Yersinia genomes had a similar global partition of protein functions, as measured by the distribution of Cluster of Orthologous Groups families. Genome to genome variation in islands with genes encoding functions such as ureases, hydrogeneases and B-12 cofactor metabolite reactions may reflect adaptations to colonizing specific host habitats. Rapid high-quality draft sequencing was used successfully to compare pathogenic and non-pathogenic members of the Yersinia genus. This work underscores the importance of the acquisition of horizontally transferred genes in the evolution of Y. pestis and points to virulence determinants that have been gained and lost on multiple occasions in the history of the genus.en_US
dc.identifier.citationChen, P.E., Cook, C., Stewart, A.C. et al. Genomic characterization of the Yersinia genus. Genome Biol 11, R1 (2010).en_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectInsertion Sequenceen_US
dc.subjectYersinia Enterocoliticaen_US
dc.subjectYersinia Pestisen_US
dc.subjectBile Salt Hydrolaseen_US
dc.subjectShort Read Archiveen_US
dc.titleGenomic characterization of the Yersinia genusen_US


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