QUANTUM DOT ENCAPSULATED VIRAL LIKE PARTICLES BASED ON ADENO- ASSOCIATED VIRUS

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dc.contributor.advisorDuncan, Greggen_US
dc.contributor.authorWolpert, Matthew Jamesen_US
dc.contributor.departmentBioengineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2020-10-10T05:37:55Z
dc.date.available2020-10-10T05:37:55Z
dc.date.issued2020en_US
dc.description.abstractAdeno-associated virus (AAV) has emerged as a leading gene-therapy candidate due to its broad tissue tropism and encouraging safety profile. Traditionally, viral biodistribution is assessed through expression of reporter genes in tissues days to weeks post-vector administration. This does not provide insight into how AAV initially distributes throughout the body which will impact its effectiveness and long-term safety. Towards this end, we optimized a strategy for assembling a viral like particle (VLP) using capsid proteins from AAV serotype 2 and incorporated a quantum dot (QD) into its core. Their uptake was visualized in human embryonic kidney (HEK) cells in vitro. Our findings enable further optimization of VLP-QD systems for AAV biodistribution analysis. In addition, extracellular vesicles (EVs) are a new class of gene-delivery vector with potential in pulmonary disease. We measured the diffusion of EVs from two cell lines in human mucus to determine their potential as an inhaled therapeutic.en_US
dc.identifierhttps://doi.org/10.13016/uuwe-8ymk
dc.identifier.urihttp://hdl.handle.net/1903/26627
dc.language.isoenen_US
dc.subject.pqcontrolledBioengineeringen_US
dc.subject.pquncontrolledAdeno-Associated Virusen_US
dc.subject.pquncontrolledextracellular vesicleen_US
dc.subject.pquncontrolledquantum doten_US
dc.subject.pquncontrolledViral like particleen_US
dc.titleQUANTUM DOT ENCAPSULATED VIRAL LIKE PARTICLES BASED ON ADENO- ASSOCIATED VIRUSen_US
dc.typeThesisen_US

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