Protein Engineering Approaches to Improve the Therapeutic Potential of Histatin-5 for Candida albicans Infections

Simple item page
dc.contributor.advisorKarlsson, Amy Jen_US
dc.contributor.authorLeissa, Jesse Altonen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2019-10-01T05:39:57Z
dc.date.available2019-10-01T05:39:57Z
dc.date.issued2019en_US
dc.description.abstractThe salivary peptide histatin 5 has been studied as a novel therapeutic to address rising drug resistance and limited therapeutics for treating infections caused by the fungal pathogen Candida albicans. While histatin 5 possesses antifungal activity, degradation from secreted aspartic proteases produced by C. albicans hinders its potential. To develop a strategy that will identify variants of histatin 5 with improved proteolytic stability, the peptide was integrated into a yeast surface display system, and the proteolytic degradation conditions were optimized to improve the resolution between proteolytically stable (K11RK17R) and proteolytically susceptible (K13L) variants. Additionally, histatin 5 and K11RK17R were embedded in polyelectrolyte multilayer films (PEMs) to investigate their ability to prevent biofilm formation on surfaces. Significant biofilm formation was prevented at high concentrations of K11RK17R, while histatin 5 encouraged biofilm formation. My results support the therapeutic potential of histatin 5 and will enable the design of improved peptide-based therapeutic approaches.en_US
dc.identifierhttps://doi.org/10.13016/3x7s-fvqg
dc.identifier.urihttp://hdl.handle.net/1903/25141
dc.language.isoenen_US
dc.subject.pqcontrolledChemical engineeringen_US
dc.subject.pquncontrolledCandida albicansen_US
dc.subject.pquncontrolledHistatin-5en_US
dc.titleProtein Engineering Approaches to Improve the Therapeutic Potential of Histatin-5 for Candida albicans Infectionsen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Leissa_umd_0117N_20225.pdf
Size:
10.82 MB
Format:
Adobe Portable Document Format
Download

Collections

UMD Theses and Dissertations
Chemical and Biomolecular Engineering Theses and Dissertations