Programmed Ribosomal Frameshifting in SARS-CoV and HIV-1

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dc.contributor.advisorDinman, Jonathan Den_US
dc.contributor.authorNeeriemer, Jessicaen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2008-04-22T16:05:41Z
dc.date.available2008-04-22T16:05:41Z
dc.date.issued2007-12-10en_US
dc.description.abstractProgrammed ribosomal frameshifting controls the ratio of two protein products made in a variety of viruses and mammalian cells. This occurs when the ribosome is translating mRNA, pauses at secondary structure, slips back one base in the 5' direction, and continues translation in a new reading frame. A series of SARS-CoV pseudoknot mutants were generated to examine important features of frameshifting, and an antibiotic was tested for its effect on HIV and SARS-CoV frameshifting. Other mutants were made in the human CCR5 gene to determine whether frameshifting occurs. It was found that mRNA stability and unpaired adenosines influence frameshifting, and increasing concentrations of the antibiotic gentamicin increases frameshifting. Moreover, CCR5, the co-receptor for HIV, contains a working frameshifting signal. This study pinpoints several antiviral targets and important factors for HIV and SARS-CoV pathogenesis.en_US
dc.format.extent1365932 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/7713
dc.language.isoen_US
dc.subject.pqcontrolledBiology, Virologyen_US
dc.subject.pqcontrolledBiology, Virologyen_US
dc.subject.pquncontrolledframeshiftingen_US
dc.subject.pquncontrolledribosomeen_US
dc.subject.pquncontrolledvirusen_US
dc.subject.pquncontrolledHIVen_US
dc.subject.pquncontrolledSARSen_US
dc.titleProgrammed Ribosomal Frameshifting in SARS-CoV and HIV-1en_US
dc.typeThesisen_US

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