Bioactivity of Ephedra: Integrating Cytotoxicity Assessment with Real-Time Biosensing

dc.contributor.advisorLo, Y. Martinen_US
dc.contributor.authorFukushima, Kazukoen_US
dc.contributor.departmentFood Scienceen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2004-08-27T05:19:01Z
dc.date.available2004-08-27T05:19:01Z
dc.date.issued2004-08-12en_US
dc.description.abstractEphedrine-type alkaloids (ETA) are major active ingredients of Ephedra, a traditional Chinese medicinal herb used to treat asthma and nasal congestion. Until recently, large amounts of Ephedra were used in dietary supplements for weight loss and athletic performance enhancement. However, indiscriminate consumption of ETA-containing products has resulted in more than 1,000 reported cases of adverse effects. The objective of this study is to evaluate bioactivity of ETA. The toxicities of (-)-ephedrine and (+)-pseudoephedrine were measured using MTT assay on human neuroblastoma (SH-SY5Y) and rat myoblastoma (H9c2 (2-1)), while the stress responses of a panel of biosensing bioluminescent Escherichia coli strains were analyzed. SH-SY5Y showed similar sensitivity to (-)-ephedrine and (+)-pseudoephedrine, while H9c2 (2-1) could differentiate the cytotoxicity of (-)-ephedrine and (+)-pseudoephedrine. The biosensing of the E. coli strains was highly sensitive to the toxicity of ETA and could yield instantaneous response. The RLU ratios dependent on the construct of strains gave unique fingerprintingm pattern of ETA.en_US
dc.format.extent1811860 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/1715
dc.language.isoen_US
dc.subject.pqcontrolledAgriculture, Food Science and Technologyen_US
dc.titleBioactivity of Ephedra: Integrating Cytotoxicity Assessment with Real-Time Biosensingen_US
dc.typeThesisen_US

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