POSITIONAL CLONING OF BROAD-SPECTRUM LEAF RUST RESISTANCE GENE, LR57, FROM AEGILOPS GENICULATA, A TERTIARY GENE POOL MEMBER OF WHEAT

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dc.contributor.advisorRawat, Nidhien_US
dc.contributor.advisorErwin, Johnen_US
dc.contributor.authorSchulden, Taylor Francisen_US
dc.contributor.departmentPlant Science and Landscape Architecture (PSLA)en_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2023-10-10T05:33:28Z
dc.date.available2023-10-10T05:33:28Z
dc.date.issued2023en_US
dc.description.abstractThe tertiary gene pool of wheat includes wild relatives like Aegilops geniculata (UUMM, 2n=4x=28) that are valuable genetic reservoirs for novel abiotic and biotic resistance genes. However, modern wheat varieties share limited genomic commonality with these gene pool members presenting barriers to recombination and genetic mapping of desirable genes. We mapped a broad-spectrum leaf rust resistance gene Lr57 located on chromosome 5Mg of Ae. geniculata using a simple but powerful methodology for high-resolution genetic mapping in tertiary gene pool members of wheat. Five gene candidates were revealed all with possible defense related functions. Strategic application of differential expression analysis, Virus-Induced Gene Silencing, and mutagenesis analysis reduced the candidate gene of interest to a singular and novel ID-NLR resistance gene containg a protein kinase, NB-Arc, and LRR domain. Using multiple strategies, validation of Lr57 candidate was completed. Gene complementation by transformation of Lr57 candidate is currently being conducted.en_US
dc.identifierhttps://doi.org/10.13016/dspace/dulw-ji7e
dc.identifier.urihttp://hdl.handle.net/1903/30901
dc.language.isoenen_US
dc.subject.pqcontrolledPlant sciencesen_US
dc.subject.pqcontrolledPlant pathologyen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pquncontrolledaegilops geniculataen_US
dc.subject.pquncontrolledcloningen_US
dc.subject.pquncontrolledLeaf rusten_US
dc.subject.pquncontrolledLr57en_US
dc.subject.pquncontrolledtertiary gene poolen_US
dc.subject.pquncontrolledwheaten_US
dc.titlePOSITIONAL CLONING OF BROAD-SPECTRUM LEAF RUST RESISTANCE GENE, LR57, FROM AEGILOPS GENICULATA, A TERTIARY GENE POOL MEMBER OF WHEATen_US
dc.typeThesisen_US

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