Acclimation and Compensating Metabolite Responses to UV-B Radiation in Natural and Transgenic Populus spp. Defective in Lignin Biosynthesis

dc.contributor.authorWong, Tiffany M.
dc.contributor.authorSullivan, Joe H.
dc.contributor.authorEisenstein, Edward
dc.date.accessioned2023-10-19T19:43:56Z
dc.date.available2023-10-19T19:43:56Z
dc.date.issued2022-08-20
dc.description.abstractPlants have evolved to protect leaf mesophyll tissue from damage caused by UV-B radiation by producing an array of UV-absorbing secondary metabolites. Flavonoids (phenolic glycosides) and sinapate esters (hydroxycinnamates) have been implicated as UV-B protective compounds because of the accumulation in the leaf epidermis and the strong absorption in the wavelengths corresponding to UV. Environmental adaptations by plants also generate a suite of responses for protection against damage caused by UV-B radiation, with plants from high elevations or low latitudes generally displaying greater adaptation or tolerance to UV-B radiation. In an effort to explore the relationships between plant lignin levels and composition, the origin of growth elevation, and the hierarchical synthesis of UV-screening compounds, a collection of natural variants as well as transgenic Populus spp. were examined for sensitivity or acclimation to UV-B radiation under greenhouse and laboratory conditions. Noninvasive, ecophysiological measurements using epidermal transmittance and chlorophyll fluorescence as well as metabolite measurements using UPLC-MS generally revealed that the synthesis of anthocyanins, flavonoids, and lignin precursors are increased in Populus upon moderate to high UV-B treatment. However, poplar plants with genetic modifications that affect lignin biosynthesis, or natural variants with altered lignin levels and compositions, displayed complex changes in phenylpropanoid metabolites. A balance between elevated metabolic precursors to protective phenylpropanoids and increased biosynthesis of these anthocyanins, flavonoids, and lignin is proposed to play a role in the acclimation of Populus to UV-B radiation and may provide a useful tool in engineering plants as improved bioenergy feedstocks.
dc.description.urihttps://doi.org/10.3390/metabo12080767
dc.identifierhttps://doi.org/10.13016/dspace/kd2h-ku7j
dc.identifier.citationWong, T.M.; Sullivan, J.H.; Eisenstein, E. Acclimation and Compensating Metabolite Responses to UV-B Radiation in Natural and Transgenic Populus spp. Defective in Lignin Biosynthesis. Metabolites 2022, 12, 767.
dc.identifier.urihttp://hdl.handle.net/1903/31080
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtPlant Science & Landscape Architectureen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectUV-B radiation
dc.subjectPopulus
dc.subjectacclimation
dc.subjectlignin
dc.subjectsecondary metabolites
dc.subjecttargeted metabolomics
dc.subjectphenylpropanoids
dc.subjectanthocyanins
dc.subjectsalicylates
dc.titleAcclimation and Compensating Metabolite Responses to UV-B Radiation in Natural and Transgenic Populus spp. Defective in Lignin Biosynthesis
dc.typeArticle
local.equitableAccessSubmissionNo

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