Nutritional and Physiological Control of Metabolic Pathways That Alter Milk Protein and Lactose Synthesis by the Mammary Gland

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dc.contributor.advisorBequette, Brian Jen_US
dc.contributor.authorSchoenberg, Katie Marieen_US
dc.contributor.departmentAnimal Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2007-02-01T20:23:44Z
dc.date.available2007-02-01T20:23:44Z
dc.date.issued2006-12-08en_US
dc.description.abstractThe objectives of this research were to develop a [U-<sup>13</sup>C]glucose tracer approach establishing the pathways and substrates for milk lactose and casein synthesis, and determine the influence of protein intake on murine mammary gland metabolism. Milk samples were collected after one, three and five days of feeding tracer (as 10% of dextrose). <sup>13</sup>C-Isotopic and isotopomer plateaus were attained by day three, establishing the time-course necessary for tracer feeding. 23% of lactose-derived glucose originated from sources other than blood glucose. Six paired (intake and pups equal) sets of lactating mice were fed either a normal (20%) or low (10%) protein diet. <sup>13</sup>C-mass isotopomer distribution (MID) in lactose-derived glucose and galactose did not differ, indicating common mammary metabolic pools. <sup>13</sup>C-MID in blood versus casein indicated significant mammary synthesis of glutamate (Normal:51%, Low:50%), alanine (Normal:32%, Low:29%), and serine (Normal:18%, Low:37%, P < 0.05), suggesting additional requirements for glucose and/or EAA for NEAA synthesis.en_US
dc.format.extent2330485 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/4213
dc.language.isoen_US
dc.subject.pqcontrolledAgriculture, Animal Culture and Nutritionen_US
dc.subject.pquncontrolledmilk synthesisen_US
dc.subject.pquncontrolledlactoseen_US
dc.subject.pquncontrolledmilk proteinen_US
dc.subject.pquncontrolledmouseen_US
dc.subject.pquncontrolledphosphoenolpyruvate carboxykinaseen_US
dc.titleNutritional and Physiological Control of Metabolic Pathways That Alter Milk Protein and Lactose Synthesis by the Mammary Glanden_US
dc.typeThesisen_US

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