Data for "A tug of war between filament treadmilling and myosin induced contractility generates actin ring"

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dc.contributor.advisorUpadhyaya, Arpita
dc.contributor.advisorPapoian, Garegin A.
dc.contributor.authorNi, Qin
dc.contributor.authorWagh, Kaustubh
dc.contributor.authorPathni, Aashli
dc.contributor.authorNi, Haoran
dc.contributor.authorVashisht, Vishavdeep
dc.contributor.authorUpadhyaya, Arpita
dc.contributor.authorPapoian, Garegin A.
dc.date.accessioned2022-08-16T19:54:27Z
dc.date.available2022-08-16T19:54:27Z
dc.date.issued2022-06-23
dc.descriptionExperimental data and simulation codes for the paper "A tug of war between filament treadmilling and myosin induced contractility generates actin ring." https://doi.org/10.1101/2021.06.06.447254en_US
dc.description.abstractIn most eukaryotic cells, actin filaments assemble into a shell-like actin cortex under the plasma membrane, controlling cellular morphology, mechanics, and signaling. The actin cortex is highly polymorphic, adopting diverse forms such as the ring-like structures found in podosomes, axonal rings, and immune synapses. The biophysical principles that underlie the formation of actin rings and cortices remain unknown. Using a molecular simulation platform, called MEDYAN, we discovered that varying the filament treadmilling rate and myosin concentration induces a finite size phase transition in actomyosin network structures. We found that actomyosin networks condense into clusters at low treadmilling rates or high myosin concentration but form ring-like or cortex-like structures at high treadmilling rates and low myosin concentration. This mechanism is supported by our corroborating experiments on live T cells, which exhibit ring-like actin networks upon activation by stimulatory antibody. Upon disruption of filament treadmilling or enhancement of myosin activity, the pre-existing actin rings are disrupted into actin clusters or collapse towards the network center respectively. Our analyses suggest that the ring-like actin structure is a preferred state of low mechanical energy, which is, importantly, only reachable at sufficiently high treadmilling rates.en_US
dc.description.sponsorshipThis work was supported by the following grants: * NSF CHE-1800418 * NSF PHY-1806903en_US
dc.identifierhttps://doi.org/10.13016/9t26-ovid
dc.identifier.urihttp://hdl.handle.net/1903/29089
dc.language.isoen_USen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtPhysicsen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectMEDYANen_US
dc.subjectsimulationen_US
dc.subjectcytoskeletonen_US
dc.subjectactinen_US
dc.titleData for "A tug of war between filament treadmilling and myosin induced contractility generates actin ring"en_US
dc.typeSoftwareen_US

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