Detecting heterogeneity in and between breast cancer cell lines

dc.contributor.authorShen, Yang
dc.contributor.authorSchmidt, B. U. Sebastian
dc.contributor.authorKubitschke, Hans
dc.contributor.authorMorawetz, Erik W.
dc.contributor.authorWolf, Benjamin
dc.contributor.authorKäs, Josef A.
dc.contributor.authorLosert, Wolfgang
dc.date.accessioned2021-07-08T14:55:25Z
dc.date.available2021-07-08T14:55:25Z
dc.date.issued2020-02-03
dc.description.abstractCellular heterogeneity in tumor cells is a well-established phenomenon. Genetic and phenotypic cell-to-cell variability have been observed in numerous studies both within the same type of cancer cells and across different types of cancers. Another known fact for metastatic tumor cells is that they tend to be softer than their normal or non-metastatic counterparts. However, the heterogeneity of mechanical properties in tumor cells are not widely studied. Here we analyzed single-cell optical stretcher data with machine learning algorithms on three different breast tumor cell lines and show that similar heterogeneity can also be seen in mechanical properties of cells both within and between breast tumor cell lines. We identified two clusters within MDA-MB-231 cells, with cells in one cluster being softer than in the other. In addition, we show that MDA-MB-231 cells and MDA-MB-436 cells which are both epithelial breast cancer cell lines with a mesenchymal-like phenotype derived from metastatic cancers are mechanically more different from each other than from non-malignant epithelial MCF-10A cells. Since stiffness of tumor cells can be an indicator of metastatic potential, this result suggests that metastatic abilities could vary within the same monoclonal tumor cell line.en_US
dc.description.urihttps://doi.org/10.1186/s41236-020-0010-1
dc.identifierhttps://doi.org/10.13016/insc-npb5
dc.identifier.citationShen, Y., Schmidt, B.U.S., Kubitschke, H. et al. Detecting heterogeneity in and between breast cancer cell lines. Cancer Converg 4, 1 (2020).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27321
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtPhysicsen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectCanceren_US
dc.subjectHeterogeneityen_US
dc.subjectSingle-cellen_US
dc.subjectMDA-MB-231en_US
dc.subjectMCF-10Aen_US
dc.subjectMDA-MB-436en_US
dc.titleDetecting heterogeneity in and between breast cancer cell linesen_US
dc.typeArticleen_US

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