Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

dc.contributor.authorTayengwa, Reuben
dc.contributor.authorSharma Koirala, Pushpa
dc.contributor.authorPierce, Courtney F.
dc.contributor.authorWerner, Breanna E.
dc.contributor.authorNeff, Michael M.
dc.date.accessioned2021-03-02T15:15:30Z
dc.date.available2021-03-02T15:15:30Z
dc.date.issued2020-11-11
dc.description.abstractThe 29-member Arabidopsis AHL gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains. AHL gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored. Transgenic plants overexpressing AtAHL20 flowered later than the wild type under both short and long days. Transcript accumulation analyses showed that 35S:AtAHL20 plants contained reduced FT, TSF, AGL8 and SPL3 mRNA levels. Similarly, overexpression of AtAHL20’s orthologue in Camelina sativa, Arabidopsis’ closely related Brassicaceae family member species, conferred a late-flowering phenotype via suppression of CsFT expression. However, overexpression of an aberrant AtAHL20 gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL19, AtAHL22 and AtAHL29. We showed via gain-of-function analysis that AtAHL20 is a negative regulator of FT expression, as well as other downstream flowering time regulating genes. A similar outcome in Camelina sativa transgenic plants overexpressing CsAHL20 suggest that this is a conserved function. Our results demonstrate that AtAHL20 acts as a photoperiod-independent negative regulator of transition to flowering.en_US
dc.description.urihttps://doi.org/10.1186/s12870-020-02733-5
dc.identifierhttps://doi.org/10.13016/5jm2-6uex
dc.identifier.citationTayengwa, R., Sharma Koirala, P., Pierce, C.F. et al. Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression. BMC Plant Biol 20, 559 (2020).en_US
dc.identifier.urihttp://hdl.handle.net/1903/26912
dc.language.isoen_USen_US
dc.publisherSpringer Nature
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtPlant Science & Landscape Architectureen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectAHLen_US
dc.subjectAHL20en_US
dc.subjectArabidopsisen_US
dc.subjectAT-hooken_US
dc.subjectFloweringen_US
dc.subjectFTen_US
dc.titleOverexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expressionen_US
dc.typeArticleen_US

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