Novel Models for Studying Trophoblast Development and Placental Pathologies

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dc.contributor.advisorTelugu, Bhanuen_US
dc.contributor.authorPence, Laramieen_US
dc.contributor.departmentMolecular and Cell Biologyen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2020-02-01T06:34:17Z
dc.date.available2020-02-01T06:34:17Z
dc.date.issued2019en_US
dc.description.abstractPlacental development begins in the mammalian blastocyst, when the first lineage specification event commits one cell population to making extraembryonic tissues, including the placenta, and commits another cell population to making the embryo proper. The mouse is an excellent animal model to study these early events and how the resulting placenta organ supports normal fetal development and a healthy pregnancy in the mother. The studies included in this Dissertation use the mouse to understand the role of long non-coding RNAs during early placental development, and to create a lineage biasing model that takes advantage of what is known about the first lineage specification event in mammals. Using expression analysis and the CRISPR/Cas9 system to create a knockout mouse strain, a placental-specific lncRNA was discovered and shown to be expressed in derivatives of the ectoplacental cone. Additionally, using the line age bias model to cause biased ablation of Hif1α in the placenta has revealed a role for fetal vs. placental contribution of resulting phenotypes.en_US
dc.identifierhttps://doi.org/10.13016/ykck-hwrk
dc.identifier.urihttp://hdl.handle.net/1903/25389
dc.language.isoenen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pqcontrolledDevelopmental biologyen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pquncontrolledCRISPRen_US
dc.subject.pquncontrolledEmbryologyen_US
dc.subject.pquncontrolledlncRNAen_US
dc.subject.pquncontrolledMouseen_US
dc.subject.pquncontrolledPlacentaen_US
dc.titleNovel Models for Studying Trophoblast Development and Placental Pathologiesen_US
dc.typeDissertationen_US

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