NOVEL METHODOLOGY FOR CALCIUM MEASUREMENTS IN CONSCIOUS MICE: AN APPLICATION TO ARTERIOLAR VASOMOTION

dc.contributor.advisorWier, Withrow Gen_US
dc.contributor.advisorChen, Yuen_US
dc.contributor.authorZviman, Adam Lyleen_US
dc.contributor.departmentBioengineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2015-09-18T05:45:44Z
dc.date.available2015-09-18T05:45:44Z
dc.date.issued2015en_US
dc.description.abstractWe developed a method employing two-photon microscopy and genetically engineered ‘Ca2+- biosensor’ mice to measure Ca2+ signaling in arteries of conscious, head-fixed mice. Arterial blood pressure was measured simultaneously via implanted telemetric pressure transducers. These methods allowed, for the first time, the study of control of arterial [Ca2+], diameter, and blood pressure by sympathetic nerve activity (SNA) as it normally exists in conscious animals. We tested the hypothesis that arterial vasomotion, observed more frequently in conscious animals than in anesthetized animals, was generated by SNA that caused synchronous Ca2+-oscillations in smooth muscle. We also measured the changes in arterial [Ca2+] that occur during the time course of experimental hypertension. Pharmacological block of SNA and isoflurane anesthesia eliminated and attenuated, respectively, vasomotion and reduced arterial [Ca2+] (320 ± 48 nM to 241 ± 20 nM and 302 ± 48 nM). This method allows for longitudinal studies of important chronic vascular pathologies.en_US
dc.identifierhttps://doi.org/10.13016/M2ZP88
dc.identifier.urihttp://hdl.handle.net/1903/16994
dc.language.isoenen_US
dc.subject.pqcontrolledPhysiologyen_US
dc.subject.pquncontrolledCardiovascularen_US
dc.subject.pquncontrolledHypertensionen_US
dc.subject.pquncontrolledPhysiologyen_US
dc.titleNOVEL METHODOLOGY FOR CALCIUM MEASUREMENTS IN CONSCIOUS MICE: AN APPLICATION TO ARTERIOLAR VASOMOTIONen_US
dc.typeThesisen_US

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