A core microbiome associated with the peritoneal tumors of pseudomyxoma peritonei

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dc.contributor.authorGilbreath, Jeremy J
dc.contributor.authorSemino-Mora, Cristina
dc.contributor.authorFriedline, Christopher J
dc.contributor.authorLiu, Hui
dc.contributor.authorBodi, Kip L
dc.contributor.authorMcAvoy, Thomas J
dc.contributor.authorFrancis, Jennifer
dc.contributor.authorNieroda, Carol
dc.contributor.authorSardi, Armando
dc.contributor.authorDubois, Andre
dc.contributor.authorLazinski, David W
dc.contributor.authorCamilli, Andrew
dc.contributor.authorTesterman, Traci L
dc.contributor.authorMerrell, D Scott
dc.date.accessioned2021-09-27T17:43:29Z
dc.date.available2021-09-27T17:43:29Z
dc.date.issued2013-07-12
dc.description.abstractPseudomyxoma peritonei (PMP) is a malignancy characterized by dissemination of mucus-secreting cells throughout the peritoneum. This disease is associated with significant morbidity and mortality and despite effective treatment options for early-stage disease, patients with PMP often relapse. Thus, there is a need for additional treatment options to reduce relapse rate and increase long-term survival. A previous study identified the presence of both typed and non-culturable bacteria associated with PMP tissue and determined that increased bacterial density was associated with more severe disease. These findings highlighted the possible role for bacteria in PMP disease. To more clearly define the bacterial communities associated with PMP disease, we employed a sequenced-based analysis to profile the bacterial populations found in PMP tumor and mucin tissue in 11 patients. Sequencing data were confirmed by in situ hybridization at multiple taxonomic depths and by culturing. A pilot clinical study was initiated to determine whether the addition of antibiotic therapy affected PMP patient outcome. We determined that the types of bacteria present are highly conserved in all PMP patients; the dominant phyla are the Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. A core set of taxon-specific sequences were found in all 11 patients; many of these sequences were classified into taxonomic groups that also contain known human pathogens. In situ hybridization directly confirmed the presence of bacteria in PMP at multiple taxonomic depths and supported our sequence-based analysis. Furthermore, culturing of PMP tissue samples allowed us to isolate 11 different bacterial strains from eight independent patients, and in vitro analysis of subset of these isolates suggests that at least some of these strains may interact with the PMP-associated mucin MUC2. Finally, we provide evidence suggesting that targeting these bacteria with antibiotic treatment may increase the survival of PMP patients. Using 16S amplicon-based sequencing, direct in situ hybridization analysis and culturing methods, we have identified numerous bacterial taxa that are consistently present in all PMP patients tested. Combined with data from a pilot clinical study, these data support the hypothesis that adding antimicrobials to the standard PMP treatment could improve PMP patient survival.en_US
dc.description.urihttps://doi.org/10.1186/1750-1172-8-105
dc.identifierhttps://doi.org/10.13016/gw1e-7ain
dc.identifier.citationGilbreath, J.J., Semino-Mora, C., Friedline, C.J. et al. A core microbiome associated with the peritoneal tumors of pseudomyxoma peritonei. Orphanet J Rare Dis 8, 105 (2013).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28022
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.relation.isAvailableAtA. James Clark School of Engineeringen_us
dc.relation.isAvailableAtChemical & Biomolecular Engineeringen_us
dc.subjectPMPen_US
dc.subjectPseudomyxoma peritoneien_US
dc.subjectPeritoneal canceren_US
dc.subjectMicrobiomeen_US
dc.subjectMicrobiotaen_US
dc.titleA core microbiome associated with the peritoneal tumors of pseudomyxoma peritoneien_US
dc.typeArticleen_US

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