Quake: quality-aware detection and correction of sequencing errors
| dc.contributor.author | Kelley, David R | |
| dc.contributor.author | Schatz, Michael C | |
| dc.contributor.author | Salzberg, Steven L | |
| dc.date.accessioned | 2013-01-10T21:13:43Z | |
| dc.date.available | 2013-01-10T21:13:43Z | |
| dc.date.issued | 2010-11-29 | |
| dc.description.abstract | We introduce Quake, a program to detect and correct errors in DNA sequencing reads. Using a maximum likelihood approach incorporating quality values and nucleotide specific miscall rates, Quake achieves the highest accuracy on realistically simulated reads. We further demonstrate substantial improvements in de novo assembly and SNP detection after using Quake. Quake can be used for any size project, including more than one billion human reads, and is freely available as open source software from http://www.cbcb.umd.edu/software/quake. | en_US |
| dc.description.uri | https://doi.org/10.1186/gb-2010-11-11-r116 | |
| dc.identifier.citation | Kelley et al. Genome Biology 2010, 11:R116 http://genomebiology.com/2010/11/11/R116 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1903/13363 | |
| dc.language.iso | en_US | en_US |
| dc.relation.isAvailableAt | College of Computer, Mathematical & Physical Sciences | en_us |
| dc.relation.isAvailableAt | Digital Repository at the University of Maryland | en_us |
| dc.relation.isAvailableAt | Biology | en_us |
| dc.relation.isAvailableAt | University of Maryland (College Park, MD) | en_us |
| dc.subject | DNA sequencing | en_US |
| dc.subject | Quake | en_US |
| dc.title | Quake: quality-aware detection and correction of sequencing errors | en_US |
| dc.type | Article | en_US |