The viability of samples collected in the BioCasade Exhaled Breath Sampler (BEBS) machine is to be measured by simulating an exhaled breath sampling event via a Collison nebulizer. There are four stages the nebulized stimulants are divided into within the BEBS system, they divide the particles based on size. The stages are labeled large, medium, small, and BioSpot and they collect particles of sizes >8.2µm, 3.3-8.2µm, 1.15-3.3µm, and <1.15µm respectively. Similar experiments have been done to examine the viability of collected samples in the BEBS system but all are done at lower collection flow rates, where this test collects at 12 lpm. In addition, this experiment collects the nebulized virus onto a hydrogel surface as opposed to previously used liquid mediums. PR8 virus will be used as the model to simulate the collection of influenza viruses. The purpose of this experiment is to recreate real-world collection data to ensure the BEBS efficacy for collecting culturable samples prior to clinical use in the EMIT-2 study.