METABOLIC ENGINEERING OF EUKARYOTIC SIGNAL TRANSDUCTION IN DROSOPHILA SCHNEIDER 2 (S2) CELL CULTURE

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dc.contributor.advisorBentley , William Een_US
dc.contributor.authorMarch, Johnen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2005-08-03T14:12:37Z
dc.date.available2005-08-03T14:12:37Z
dc.date.issued2005-04-20en_US
dc.description.abstractEukaryotic cell culture presents metabolic engineers with opportunities not found in bacterial systems. The cell cycle enables higher order organisms to continually check their growth conditions, taking internal inventory of their general metabolic state before committing to DNA replication or cell division. Cell cycle check points and growth stimuli comprise an intricate feedback loop. Similarly, insulin signaling works to insure cells do not overproliferate when taking advantage of nutrient availability within a developing multicellular organism. However, the insulin signaling pathway is present in Drosophila S2 cells as well as whole flies, and may control growth in cell culture. The work described here exploited the insulin signaling pathway and the cell cycle for enhanced cell growth and heterologous protein expression. By using the relatively new approach of RNA interference (RNAi) gene silencing, key intermediates involved in growth and cell cycling were interrupted. This work demonstrates both the potential of RNAi and the utility of controlling cellular signaling pathways as new and powerful tools in metabolic engineering.en_US
dc.format.extent696696 bytes
dc.format.extent696696 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/2450
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pquncontrolledRNAien_US
dc.subject.pquncontrolledTSC1en_US
dc.subject.pquncontrolledCyclin Een_US
dc.subject.pquncontrolledtubular sclerosisen_US
dc.subject.pquncontrolledcell cycleen_US
dc.titleMETABOLIC ENGINEERING OF EUKARYOTIC SIGNAL TRANSDUCTION IN DROSOPHILA SCHNEIDER 2 (S2) CELL CULTUREen_US
dc.typeDissertationen_US

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