Resistance to Ionizing Radiation and Oxidative Stress in Halobacterium salinarum NRC-1

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dc.contributor.advisorDinman, Jonathanen_US
dc.contributor.authorRobinson, Courtney Kathrynen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2010-02-19T07:06:29Z
dc.date.available2010-02-19T07:06:29Z
dc.date.issued2009en_US
dc.description.abstractOxidative stress results from environmental challenges that cause unchecked production of reactive oxygen species (ROS). We analyzed the cellular damage and stress response of the extremophile <italic>Halobacterium salinarum</italic> NRC-1 exposed to chemical oxidants and to ionizing radiation (IR). In contrast to IR, cellular damage from H<sub>2</sub>O<sub>2</sub> and superoxide suggested that cell death resulted from interference with major metabolic pathways rather than generalized oxidative lesions. We found that essential ROS scavenging enzymes were not necessary for <italic>H. salinarum</italic> NRC-1 survival to IR. Protection assays using enzyme-free cellular extracts from <italic>H. salinarum</italic> NRC-1 demonstrated high level of protection for protein activity but not for DNA integrity against IR. Biochemical analysis of the extracts underlined an essential role in ROS scavenging for specific nucleosides and MnPO<sub>4</sub> complexes. These studies contributed novel findings on the critical role played by non-enzymatic systems in IR resistance in <italic>H. salinarum</italic> NRC-1.en_US
dc.identifier.urihttp://hdl.handle.net/1903/9991
dc.subject.pqcontrolledBiology, Molecularen_US
dc.subject.pquncontrolledArchaeaen_US
dc.subject.pquncontrolledHalobacteriumen_US
dc.subject.pquncontrolledIonizing radiationen_US
dc.subject.pquncontrolledOxidative stressen_US
dc.subject.pquncontrolledRadiation resistanceen_US
dc.subject.pquncontrolledROS scavengingen_US
dc.titleResistance to Ionizing Radiation and Oxidative Stress in Halobacterium salinarum NRC-1en_US
dc.typeThesisen_US

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