THE ROLE OF AURORA KINASE IN THE DEVELOPING MALE GAMETOPHYTE OF MARSILEA VESTITA

dc.contributor.advisorCooke, Todden_US
dc.contributor.authorBarnes, Alisha Nicoleen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2018-07-17T05:47:09Z
dc.date.available2018-07-17T05:47:09Z
dc.date.issued2018en_US
dc.description.abstractThe development of the male gametophyte of Marsilea vestita occurs via an ordered sequence of cellular events, which result in the formation of 32 motile sperm. The blepharoplast is a subcellular structure that arises de novo at ~3.5 hours following microspore hydration and functions as a microtubule organizing center during the final mitotic division. I investigated the roles of aurora kinases in the formation and maturation of the blepharoplast. Three unique aurora kinase isoforms were found in the transcriptome of the male gametophyte, each with a unique N-terminal sequence and expression pattern. RNAi knockdowns of each isoform resulted in different stages of developmental arrest, and the absence of blepharoplasts, thereby suggesting that each isoform has a unique function. Centrin phosphomimics acted to stabilize centrin, enabling centrin aggregates to form. My results suggest that each isoform of aurora kinase plays an important role in male gametophyte development in Marsilea.en_US
dc.identifierhttps://doi.org/10.13016/M2Z892J3Z
dc.identifier.urihttp://hdl.handle.net/1903/20827
dc.language.isoenen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledAurora Kinaseen_US
dc.subject.pquncontrolledCentrinen_US
dc.subject.pquncontrolledMarsileaen_US
dc.titleTHE ROLE OF AURORA KINASE IN THE DEVELOPING MALE GAMETOPHYTE OF MARSILEA VESTITAen_US
dc.typeThesisen_US

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