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Harvesting life's energy: increase in the aerotolerence of the electrogenic anaerobe geobacter sulfurreducens due to over-expression of superoxide dismutase and catalase

dc.contributor.authorAxe, Jennifer
dc.contributor.authorBillmyre, R. Blake
dc.contributor.authorDuty, Kevin Heffner
dc.contributor.authorHitz, Greg
dc.contributor.authorTrager, Lauren
dc.contributor.authorWeatherford, Allison
dc.date.accessioned2009-05-20T14:54:11Z
dc.date.available2009-05-20T14:54:11Z
dc.date.issued2009-05
dc.identifier.urihttp://hdl.handle.net/1903/9086
dc.descriptionGemstone Team iGEM
dc.description.abstractGeobacter-based microbial fuel cells are becoming increasingly viable as a source of alternative energy. Current research and commercial application have been slowed by the inability of Geobacter species to tolerate the presence of oxygen. Oxidative stress protection enzymes normally play a key role in protecting cells from oxygen damage. This project hypothesizes that the over-expression of two important oxidative stress protection enzymes, catalase and superoxide dismutase, can increase aerotolerance. These genes were amplified from the genome of Geobacter sulfurreducens and cloned into plasmid pRG5 behind the ptaclac promoter. This plasmid was transformed into both E. coli and G. sulfurreducens to examine oxygen tolerance, gene expression, and enzyme activity. Preliminary data show increases in enzyme activity in E. coli and an increase in aerotolerance in G. sulfurreducens for both genes. This seems to be the first example of an increase in an obligate anaerobe's aerotolerance due to the intentional introduction of oxidative stress genes.en
dc.format.extent5424069 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.subjectalternative energyen
dc.subjectoxidative stress protection enzymesen
dc.subjectGemstone Team iGEM
dc.titleHarvesting life's energy: increase in the aerotolerence of the electrogenic anaerobe geobacter sulfurreducens due to over-expression of superoxide dismutase and catalaseen
dc.typeThesisen
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtGemstone Program, University of Maryland (College Park, Md)


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