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FUNCTIONAL CHARACTERIZATION OF THE INTERACTION OF HEPATITIS E VIRUS ORF3 PRODUCT WITH THE CYTOSKELETON

dc.contributor.advisorZhang, Yan-Jinen_US
dc.contributor.authorKannan, Harilakshmien_US
dc.date.accessioned2009-03-24T05:32:20Z
dc.date.available2009-03-24T05:32:20Z
dc.date.issued2008en_US
dc.identifier.urihttp://hdl.handle.net/1903/8969
dc.description.abstractHepatitis E virus (HEV) causes several outbreaks of hepatitis in humans. Many aspects of HEV pathogenesis are not well understood. The HEV ORF3 product (henceforth known as vp13) is a multifunctional protein essential for infection of animals. To better understand the vp13 functions, this study was performed. We observed that vp13 protein was associated with the microtubules (MT) in transfected cells. Mutational studies revealed that both hydrophobic domains at the N-terminal region of vp13 are required for the vp13-MT interaction. Our studies also showed that HEV vp13 protein increased the stability of the MT, activated the apoptotic pathway, and, increased the levels of tumor suppressor gene p53 and its downstream effector p21Cip/WAF1 in the transfected cells. However, no noticeable effect on cell survival was observed. These results indicated that HEV vp13 protein may act as a viral regulatory protein.en_US
dc.format.extent2874403 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleFUNCTIONAL CHARACTERIZATION OF THE INTERACTION OF HEPATITIS E VIRUS ORF3 PRODUCT WITH THE CYTOSKELETONen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentVeterinary Medical Scienceen_US
dc.subject.pqcontrolledBiology, Veterinary Scienceen_US
dc.subject.pqcontrolledAgriculture, Animal Pathologyen_US
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.subject.pquncontrolledCytoskeletonen_US
dc.subject.pquncontrolledHepatitis E virusen_US
dc.subject.pquncontrolledMicrotubulesen_US
dc.subject.pquncontrolledORF3en_US


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