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RNA packaging and gene delivery using Tobacco mosaic virus pseudo virions

dc.contributor.advisorBentley, Williamen_US
dc.contributor.authorHung, Chi-Weien_US
dc.date.accessioned2008-06-20T05:37:14Z
dc.date.available2008-06-20T05:37:14Z
dc.date.issued2008-04-28en_US
dc.identifier.urihttp://hdl.handle.net/1903/8175
dc.description.abstractRNA interference (RNAi) has emerged as a powerful tool for the study of gene function and post-transcriptional regulation. However, the lack of a proper delivery system for RNAi is a major problem for its application as a therapeutic agent. In this study, <em>Tobacco mosaic virus</em> (TMV) is utilized as an RNAi carrier for gene delivery into mammalian and insect cells. The self assembly and disassembly of TMV is investigated to create chimeric viruses for gene delivery. The origin of assembly sequence (OAS) within the TMV RNA initiates its association with coat protein through a unique hairpin structure. Studies in this dissertation show that by incorporating TMV OAS into an RNA of interest, the RNA can assemble into "pseudo-virions" by the virus coat protein. The length of the pseudo-virions changed in proportion with the size of the RNA. To deliver the RNA to the targeted cells, virions are further surface-modified with synthetic cell-penetrating peptides to facilitate cell endocytosis. Two genes were selected as targets: 1) EGFP as a visual marker and 2) Cyclin E for control of cell cycle. EGFP is expressed in a transient expression experiment using a plasmid vector, pEFGP-N1. Cyclin E is regulated endogenously in High FiveTM cells, and its translation is targeted using the pseudo virions. Pseudo-virions targeting EGFP RNA (antisense EGFP) are able to suppress transient EGFP production by 61% whereas pseudo virions targeting cyclin E (antisense cycE) are capable of arresting cells at G1 phase. This RNA packaging system protects packaged RNA and provides a means of delivering RNAi constructs into various host cells.en_US
dc.format.extent2646129 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleRNA packaging and gene delivery using Tobacco mosaic virus pseudo virionsen_US
dc.typeDissertationen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentChemical Engineeringen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledEngineering, Biomedicalen_US
dc.subject.pqcontrolledBiology, Virologyen_US
dc.subject.pquncontrolledTobacco mosaic virusen_US
dc.subject.pquncontrolledgene deliveryen_US
dc.subject.pquncontrolledRNA virusen_US
dc.subject.pquncontrolledRNA interferenceen_US
dc.subject.pquncontrolledsiRNAen_US
dc.subject.pquncontrolledgene therapyen_US


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