Method for Identifying Splice Sites and Translational Start Sites in Eukaryotic mRNA
Method for Identifying Splice Sites and Translational Start Sites in Eukaryotic mRNA
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Publication or External Link
Date
1997
Authors
Salzberg, Steven L.
Advisor
Citation
A Method for Identifying Splice Sites and Translational Start Sites in Eukaryotic mRNA. S.L. Salzberg. Computer Applications in the Biosciences (CABIOS) 13:4 (1997), 365-376.
DRUM DOI
Abstract
This paper describes a new method for determining the
consensus sequences that signal the start of donor translation
and the boundaries between exons and introns (donor and acceptor sites) in eukaryotic mRNA. The method
takes into account the dependencies between adjacent
bases, in contrast to the usual technique of considering
each position independently. When coupled with a dynamic program to compute the most likely sequence, new
consensus sequences emerge. The consensus sequence information is summarized in conditional probability matrices which, when used to locate signals in uncharacterized genomic DNA, have greater sensitivity and specificity than conventional matrices. Species-specific versions of these matrices are especially effective at distinguishing true and false sites.