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Evaluation of Lef1 Transcription Factor in Mammalian Preimplantation Embryos

dc.contributor.advisorKeefer, Carol Len_US
dc.contributor.authorMeece, Ashley Elaineen_US
dc.date.accessioned2007-09-28T15:03:58Z
dc.date.available2007-09-28T15:03:58Z
dc.date.issued2007-09-14en_US
dc.identifier.urihttp://hdl.handle.net/1903/7420
dc.description.abstractThe first lineage differentiation of cells during pre-implantation embryo development is critical for continued embryonic growth. Though several transcription factors (TFs) have been identified that are involved in this transition, a specific mechanism of regulation has yet to be determined. Previous studies in our laboratory have shown the TF Lef1 is involved in differentiation of mouse embryonic stem cells into trophoblastic stem cells through the Wnt signaling pathway. Lef1 is known to be involved in lineage determination of adult skin stem cells. As we observed that two isoforms of Lef1 were expressed at different stages of pre-implantation development, we hypothesized that Lef1 was interacting with the other well-established lineage differentiation TFs, Nanog, Oct4, and Cdx2, in these early embryos. At the blastocyst stage, no significant changes in mRNA expression were seen when siRNAs, specifically designed to knockdown Lef1 expression, were injected in early embryos; however, knockdown of Lef1 did not interfere with blastocyst formation.en_US
dc.format.extent1299365 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleEvaluation of Lef1 Transcription Factor in Mammalian Preimplantation Embryosen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentAnimal Sciencesen_US
dc.subject.pqcontrolledAgriculture, Animal Culture and Nutritionen_US
dc.subject.pqcontrolledBiology, Molecularen_US
dc.subject.pquncontrolledembryoen_US
dc.subject.pquncontrolledLef1en_US
dc.subject.pquncontrolledsiRNAen_US
dc.subject.pquncontrolledbovineen_US


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