Skip to content
University of Maryland LibrariesDigital Repository at the University of Maryland
    • ログイン
    アイテム表示 
    •   ホーム
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • アイテム表示
    •   ホーム
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • アイテム表示
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Characterization of Chicken CAT-2 Isoforms

    Thumbnail
    閲覧/開く
    umi-umd-4798.pdf (2.081Mb)
    No. of downloads: 913

    日付
    2007-08-20
    著者
    Kirsch, Sandra B
    Advisor
    Hamza, Iqbal
    Humphrey, Brooke D
    Metadata
    アイテムの詳細レコードを表示する
    抄録
    Lysine and arginine transport is primarily mediated by cationic amino acid transporters (CATs) in cells. The chicken CAT-2 (cCAT-2) transcript is alternatively spliced to three isoforms. Transcriptional and cellular localization experiments were utilized to study their regulation. The mRNA abundance of cCAT-2 isoforms was estimated in body tissues, and although differentially expressed, all tissues expressed each cCAT-2 isoform gene, indicating that alternative splicing was not tissue-specific. Both cCAT-2A and cCAT-2B proteins localized to the plasma membrane and cCAT-2C protein was retained in the cytosol. Chicken CAT-2A functions as a low affinity transporter with specificity for lysine and arginine. Chicken CAT-2B and cCAT-2C transporter functions were not detectable. Our data indicates that CAT-2 transporters are conserved in non-mammalian vertebrates, but cCAT-2 isoforms differ in their tissue distribution and transporter function from previously characterized CAT-2 transporters. These results also indicate a mechanism by which additional dietary lysine and arginine contribute to increased protein accretion in muscle tissue.
    URI
    http://hdl.handle.net/1903/7383
    Collections
    • Animal & Avian Sciences Theses and Dissertations
    • UMD Theses and Dissertations

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    ブラウズ

    リポジトリ全体コミュニティ/コレクション公開日著者タイトル主題このコレクション公開日著者タイトル主題

    登録利用者

    ログイン登録
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility